Nova-ST Spatial Transcriptomics protocol

By Suresh Poovathingal, PhD, Kristofer Davie and Stein Aerts, PhD
View Published Protocol

Output Details

Description
Nova-ST is an open-source, high-resolution sequencing-based spatial transcriptomics workflow. This method gives comparable resolution to BGI Stereoseq, SeqScope & PIXEL seq. Nova-ST is derived from dense nano-patterned randomly barcoded Illumina NovaSeq 6000 S4 sequencing flow cells. More details in the Nova-ST pre-print. Nova-ST enables customized, low-cost, flexible, and high-resolution spatial profiling of a broad range of tissue section sizes (up to 10mm x 8 mm). In this protocol, we provide detailed step-by-step resource for implementing the Nova-ST spatial transcriptomics workflow in your lab. Bioinformatics and data analysis workflows are detailed in: https://github.com/aertslab/Nova-ST. For any protocol-related or data analysis clarifications, you can reach out to us via [email protected].
Identifier (DOI)
10.17504/protocols.io.3byl4925jgo5/v1
Tags
  • Spatial transcriptomics
Team
Team Voet
Program
Collaborative Research Network
Theme
PD Functional Genomics

Meet the Authors

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