pAAV_CbH_SIO_SyGCaMP8s_p2a_mRuby

Output Details

This constructs links GCaMP8s to Synaptophysin and directs the fusion protein to presynaptic sites. It also produces a cytoplasmic red fluorescence protein (mRuby) via the self-splicing peptide 2A. It is mainly used to longitudinally image synaptic activity with 2-Photon at 980nm and visualize axons at 1080nm in vivo.
Tags
  • AAV
  • AAV (Adenoassociated Virus)
  • Live-imaging
  • Neuroaxonal damage
  • Two-Photon Microscopy