Phosphatome-wide siRNA screen in 3T3 cells

View Published Protocol

Output Details

Description
This protocol describes how we performed a phosphatome-wide siRNA screen in 3T3 cells to look for LRRK2-counteracting phosphatases for pRab12 and pRab10.
Identifier (DOI)
10.17504/protocols.io.36wgqdxr5vk5/v1
Team
Team Alessi
Program
Collaborative Research Network
Theme
PD Functional Genomics

Related Research

PPM1M, a LRRK2-counteracting, phosphoRab12-preferring phosphatase with potential link to Parkinson’s disease

LRRK2 phosphorylates Rab GTPases linked to Parkinson's disease. PPM1M identified as a phosphatase reversing Rab phosphorylation, crucial in LRRK2 pathway. PPM1M mutation found in Parkinson's patients, suggesting a new therapeutic target.

View Article

Dynamic basal ganglia output signals license and suppress forelimb movements

Basal ganglia is fundamental to motor control and dysfunction is linked to motor deficits. Our results demonstrate the existence and function of highly specific and temporally precise movement representations in basal ganglia output circuitry.

View Article

Mouse Stereotaxic Surgery-Arber Lab

This protocol describes the steps for performing stereotaxic surgery in mice. It is applicable to intracranial injections (e.g. virus, drug) and placement of implants (e.g. optical fibers, electrode arrays) into targeted regions of mouse brains.

View Protocol
View More Outputs