Key Personnel: Team Hurley,
University of California, Berkeley
Authors determine cryo-EM structure of human ULK1C core and its complex with PI3KC3-C1. ULK1C core undergoes a rearrangement from 2:1:1 to 2:2:2 stoichiometry, suggesting a structural mechanism for autophagy initiation.
This protocol describes a standard procedure for subcloning of genotype-confirmed human pluripotent stem cells (hPSCs).
This protocol describes the restriction cloning strategy.
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