This paper describes the self-assembling synuclein (SAS) system, a genetically-encoded tool for inducing temporally-controllable, tunable PD pathology in a wide range of in vitro and in vitro models.
Data includes immunohistochemistry images, two-photon images of patched neurons, whole-cell recordings for somatic excitability and Sr2+-oEPSC, spine density imaging, ACh sensor imaging, and dendritic excitability linescan data.
Liqiang Chu et al found decreased excitability in these neurons in a Parkinson's mouse model. A simulation using NetPyNE was created to study the impact of the changed PT5B neuron on motor cortex spiking dynamics.
