Production of α-synuclein preformed fibrils (PFF)

View Published Protocol

Output Details

Description
This protocol outlines the procedure to produce preformed fibrils (PFF). It has been adapted from Volpicelli-Daley et al., 2014.
Identifier (DOI)
10.17504/protocols.io.dm6gp3nm5vzp/v1
Tags
  • Alpha-synuclein
  • Other
  • Pre-formed fibrils (PFFs)
Team
Team Kaplitt
Program
Collaborative Research Network
Theme
Circuitry and Brain-Body Interactions

Related Research

Gut-Initiated Alpha Synuclein Fibrils Drive Parkinson’s Disease Phenotypes: Temporal Mapping of non-Motor Symptoms and REM Sleep Behavior Disorder

Propagation of αSyn from the gut-to-the-brain induces RBD and several non-motor and motor phenotypes of Parkinson’s disease.

View Article

α-Synuclein Promotes Neuronal Dysfunction and Death by Disrupting the Binding of Ankyrin to β-Spectrin

α-Synuclein plays a key role in the pathogenesis of Parkinson’s disease and related disorders, but critical interacting partners and molecular mechanisms mediating neurotoxicity are incompletely understood. We show that α-synuclein binds…

View Article

Protocol for CRISPR-based manipulation and visualization of endogenous α-synuclein in cultured mouse hippocampal neurons

Protocol for depleting or tagging endogenous α-syn using virally-delivered CRISPR-Cas9 in hippocampal neurons

View Protocol
View More Outputs
Aligning Science Across Parkinson's
Powered by  GDPR Cookie Compliance
Privacy Overview

This website uses cookies so that we can provide you with the best user experience possible. Cookie information is stored in your browser and performs functions such as recognising you when you return to our website and helping our team to understand which sections of the website you find most interesting and useful.