Proteomics workflow for whole cell lysate, endosome, and lysosome fractions

By Frances Hundley, PhD and J. Wade Harper
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Output Details

Description

We present a protocol for sample preparation for LC-MS analysis of whole cell lysates and for lysosomal and endosomal fractions purified by Lyso-IP and Endo-IP. Protocols for purification of lysosomes and endosomes is provided in protocol dx.doi.org/10.17504/protocols.io.byi9puh6 using cells that express endogenously tagged TMEM192-HA and stably expressing FLAG-EEA1 as descrbed in dx.doi.org/10.17504/protocols.io.byi7puhn.

Identifier (DOI)
10.17504/protocols.io.bys6pwhe
Tags
  • Endosomes
  • Lysate preparation
  • Lysosomes
  • Mass spectrometry
  • Proteomics
  • Sample preparation
Team
Team Harper
Program
Collaborative Research Network
Theme
PD Functional Genomics

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