Purification of FIP200 CTR-GFP

By Eleonora Turco and Elias Adriaenssens, PhD
View Published Protocol

Output Details

Description
This protocol describes how to express and purify GFP-FIP200 (CTR).
Identifier (DOI)
10.17504/protocols.io.j8nlk8866l5r/v1
Team
Team Hurley
Program
Collaborative Research Network
Theme
PD Functional Genomics

Meet the Authors

  • User avatar fallback logo

    Eleonora Turco

  • User avatar fallback logo

    Elias Adriaenssens, PhD

    Key Personnel: Team Hurley, 

    Max F. Perutz Laboratories

Related Research

Reconstitution of BNIP3/NIX-mediated autophagy reveals two pathways and hierarchical flexibility of the initiation machinery

During selective autophagy transmembrane cargo receptors can trigger autophagy by recruiting different complexes and utilizing multiple pathways. This flexibility in autophagy initiation has important therapeutic implications.

View Article

FISH CODETECTION

DIG and Fluorescein labeled riboprobes Fluorescent in situ hybridization Frozen brain sections

View Protocol

How Membrane Contact Sites Shape the Phagophore

Phagophores form membrane contact sites with organelles like the vacuole, ER, and lipid droplets. In situ imaging techniques, such as cryo-CLEM, provide valuable insights into these interactions, aiding in understanding autophagosome biogenesis.

View Article
View More Outputs