RNA In situ hybridization (ISH) using RNAscope®

By Yue Sun, PhD and Jun Ding, PhD
View Published Protocol

Output Details

Description
This protocol outlines the procedures for RNA in situ hybridization to simultaneously detect up to three targets using the RNAscope® Multiplex Fluorescent v2 assay (ACD). The steps adhere to the manufacturer's guidelines (Doc. No: 323100-USM) with custom modifications optimized for fixed frozen mouse brain sections.
Identifier (DOI)
10.17504/protocols.io.kxygxw25ov8j/v1
Tags
  • Ex Vivo
  • In Situ
  • in situ RNA analytics
  • Mouse
  • RNA
Team
Team Surmeier
Program
Collaborative Research Network
Theme
Circuitry and Brain-Body Interactions

Meet the Authors

  • User avatar fallback logo

    Yue Sun, PhD

    Key Personnel: Team Surmeier, 

    Stanford University

  • User avatar fallback logo

    Jun Ding, PhD

    Collaborating PI: Team Surmeier, 

    Stanford University

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