Saliva metagenomic data related to “Evaluation of an Adapted Semi-Automated DNA Extraction for Human Salivary Shotgun Metagenomics”

By Victoria Meslier, PhD, Elisa Menozzi, MD, PhD, Aymeric David, Christian Morabito, MSc, Sara Lucas Del Pozo, MD, Alexandre Famechon, Janet North, Benoit Quinquis, Sofia Koletsi, MSc, Jane Macnaughtan, PhD, Roxana Mezabrovschi, BSc, Stanislav Ehrlich, PhD, Anthony Schapira, PhD and Mathieu Almeida, PhD
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Output Details

Description
In this study, we proposed a semi-automated DNA extraction protocol for human salivary samples collected with a commercial kit, and compared the outcomes with the DNA extraction recommended by the manufacturer. This study demonstrates that our semi-automated protocol is suitable for shotgun metagenomic analysis, by significantly producing higher DNA fragment sizes, while allowing for improved sample treatment logistics with reduced technical variability and without compromising the structure of the oral microbiome.
Tags
  • Antemortem
  • Human
  • Human fecal samples
  • Metagenome
Team
Team Schapira
Program
Collaborative Research Network
Theme
Neuro-Immune Interactions

Meet the Authors

  • Victoria Meslier, PhD

    Key Personnel: Team Schapira, 

    National Research Institute for Agriculture, Food and Environment

  • Elisa Menozzi, MD, PhD

    Key Personnel: Team Schapira, 

    University College London

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    Aymeric David

  • Christian Morabito, MSc

    Key Personnel: Team Schapira, 

    National Research Institute for Agriculture, Food and Environment

  • Sara Lucas Del Pozo, MD

    Key Personnel: Team Schapira, 

    University College London

  • User avatar fallback logo

    Alexandre Famechon

  • User avatar fallback logo

    Janet North

  • User avatar fallback logo

    Benoit Quinquis

  • Sofia Koletsi, MSc

    Project Manager: Team Schapira, 

    University College London

  • User avatar fallback logo

    Jane Macnaughtan, PhD

    Collaborating PI: Team Schapira, 

    University College London

  • User avatar fallback logo

    Roxana Mezabrovschi, BSc

    Key Personnel: Team Schapira, 

    University College London

  • Stanislav Ehrlich, PhD

    Collaborating PI: Team Schapira, 

    National Research Institute for Agriculture, Food and Environment

  • Anthony Schapira, PhD

    Lead PI (Core Leadership): Team Schapira, 

    University College London

  • Mathieu Almeida, PhD

    Co-PI (Core Leadership): Team Schapira, 

    INRAE (National Research Institute for Agriculture, Food and the Environment)

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