Saliva metagenomic data related to “Evaluation of an Adapted Semi-Automated DNA Extraction for Human Salivary Shotgun Metagenomics”

Output Details

In this study, we proposed a semi-automated DNA extraction protocol for human salivary samples collected with a commercial kit, and compared the outcomes with the DNA extraction recommended by the manufacturer. This study demonstrates that our semi-automated protocol is suitable for shotgun metagenomic analysis, by significantly producing higher DNA fragment sizes, while allowing for improved sample treatment logistics with reduced technical variability and without compromising the structure of the oral microbiome.
Tags
  • Antemortem
  • Human
  • Human fecal samples
  • Metagenome

Meet the Authors

  • Victoria Meslier, PhD

    Key Personnel: Team Schapira

    National Research Institute for Agriculture, Food and Environment

  • Elisa Menozzi, MD, PhD

    Key Personnel: Team Schapira

    University College London

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    Aymeric David

  • Christian Morabito, MSc

    Key Personnel: Team Schapira

    National Research Institute for Agriculture, Food and Environment

  • Sara Lucas Del Pozo, MD

    Key Personnel: Team Schapira

    University College London

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    Alexandre Famechon

  • User avatar fallback logo

    Janet North

  • User avatar fallback logo

    Benoit Quinquis

  • Sofia Koletsi, MSc

    Project Manager: Team Schapira

    University College London

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    Jane Macnaughtan, PhD

    Collaborating PI: Team Schapira

    University College London

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    Roxana Mezabrovschi, BSc

    Key Personnel: Team Schapira

    University College London

  • Stanislav Ehrlich, PhD

    Collaborating PI: Team Schapira

    National Research Institute for Agriculture, Food and Environment

  • Anthony Schapira, PhD

    Lead PI (Core Leadership): Team Schapira

    University College London

  • Mathieu Almeida, PhD

    Co-PI (Core Leadership): Team Schapira

    INRAE (National Research Institute for Agriculture, Food and the Environment)