Authors determine cryo-EM structure of human ULK1C core and its complex with PI3KC3-C1. ULK1C core undergoes a rearrangement from 2:1:1 to 2:2:2 stoichiometry, suggesting a structural mechanism for autophagy initiation.
Inherited mutations in VPS35 and LRRK2 cause Rab GTPase hyperphosphorylation. TMEM55B on lysosomes interacts with RILPL1 through a specific motif, forming complexes with various proteins, revealing new insights into lysosomal function.
Phagophores form membrane contact sites with organelles like the vacuole, ER, and lipid droplets. In situ imaging techniques, such as cryo-CLEM, provide valuable insights into these interactions, aiding in understanding autophagosome biogenesis.
