Stable integration of piggyBac plasmids into U2OS cells

Description

Generation of stably transfected U2OS human osteosarcoma cell line with piggyBac constructs (e.g., piggyBac-rtTA(#4)-SNCA-A53T-sfGFP-IRES-mKate2-puro to express transgenic SNCA). The established cell line overexpresses the transgene cloned in the piggyBac construct (e.g., alpha-synuclein) by doxycycline induction.

Identifier (DOI)

10.17504/protocols.io.14egn618ql5d/v1

Team

Team Studer

Program

Collaborative Research Network

Theme

PD Functional Genomics

Meet the Authors

Isabel Lam, PhD

Key Personnel: Team Studer,

Brigham and Women's Hospital
Alain Ndayisaba, MD

Key Personnel: Team Studer,

Brigham and Women's Hospital
Vikram Khurana, MD

Co-PI (Core Leadership): Team Studer,

Harvard University

Stable integration of piggyBac plasmids into U2OS cells

Output Details

Meet the Authors

Isabel Lam, PhD

Alain Ndayisaba, MD

Vikram Khurana, MD

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