Synaptic analysis workflow using SEQUIN

Output Details

Identification and quantification of synaptic loci is a critically important question for numerous neuroscience experiments. This protocol provides for the analysis of super-resolution images acquired on a Zeiss Airyscan microscope. Endpoints provided by this analysis workflow include identification of synaptic loci, quantification of a third label in the proximity of identified synaptic loci, and localization of identified synaptic loci to labeled neurites.
Tags
  • Immunofluorescence
  • Immunohistochemistry
  • In Vitro
  • Mouse

Meet the Authors

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    Andrew Sauerbeck, PhD

    Key Personnel: Team Biederer

    Washington University Medical Center

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    Terrance Kummer, MD, PhD

    Collaborating PI: Team Biederer

    Washington University Medical Center