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Untargeted metabolomics of Tagless Lyso-IP
Output Details
Description
Lysosomal biology is increasingly implicated in neurodegenerative diseases and health. It has traditionally been difficult to profile the metabolomic homeostasis of the lysosome in disease states. To overcome this challenge, we have developed the Tagless Lyso-IP method to rapidly prepare lysosome enriched samples from human peripheral blood. This protocol details the processing and untargeted analysis of polar metabolites derived using the Tagless Lyso-IP method.
Identifier (DOI)
10.17504/protocols.io.kxygx3jm4g8j/v1