H9 ES AAVS-NGN2; FBXO7-/-
By savannah onH9 ES cells with AAVS1-NGN2 in which FBXO7 has been deleted using CRISPR.
pCAG-TSF-ATG13 (363-517)
By savannah onMammalian expression of TwinStrep-Flag tagged ATG13 (363-517).
Plasmid: V5-KANSL1 pLVX-EF1α-IRES-Puro
By savannah onHuman KANSL1 cDNA with N-terminal V5 tag cloned into the pLVX-EF1α-IRES-Puro plasmid (EcoRI and SpeI restriction sites) from Clontech, Takara Bio.
pCAG-TSF-ATG13 (450-517)-ULK1 (836-1050)
By savannah onMammalian expression of ATG13 C-terminal and ULK1 MIT domain fusion complex.
pGEX-4T1-GST-Thrombin-TEV-EGFP-hNEMO
By savannah onHuman p62 with silent mutation for sip62 #5 (Thermo Scientific). Internal reference: SMC516 Plasmid for the expression of GFP labelled NEMO (SMC Internal No. 1977).
Plasmid: pLV[Exp]-EF1A>V5/hKAT8[NM_032188.3]:IRES:Puro
By savannah onHuman KAT8 gene with N-terminal V5 tag cloned into the pLV plasmid backbone under a EF1a promoter and including IRES puromycin selection cassette by Vectorbuilder.
Lentivirus plasmids for sgRNA: pLV[Exp]-U6>NT-Seq1-hPGK>mApple
By savannah onPlasmid vector encoding a non-targeting sgRNA sequence under a U6 promoter and a mApple fluorescent reporter. Generated by Vectorbuilder in the pLV backbone
Lentivirus plasmid: pLV[Exp]-U6>KAT8_P1_Seq1-hPGK>mApple
By savannah onPlasmid vector encoding a sgRNA sequence which targets the human PINK1 promoter for CRISPRi, under a U6 promoter and a mApple fluorescent reporter. Generated by Vectorbuilder in the pLV backbone.
Lentivirus plasmid: pLV[Exp]-U6>KAT8_P1_Seq1-hPGK>mApple
By savannah onPlasmid vector encoding a sgRNA sequence which targets the human KAT8 promoter for CRISPRi, under a U6 promoter and a mApple fluorescent reporter. Generated by Vectorbuilder in the pLV backbone.
Lentivirus plasmid: pLV[Exp]-U6>KANSL1_P1_Seq1-hPGK>mApple
By savannah onPlasmid vector encoding a sgRNA sequence which targets the KANSL1 promoter from CRISPRi, under a U6 promoter and a mApple fluorescent reporter. Generated by Vectorbuilder in the pLV backbone.