Lab Resource Archive

Plasmids for expression of TMEM192 (LysoTag) in human and mouse cells.

Plasmids for expression of TMEM115 (GolgiTag) in human and mouse cells.

mCh-Miro1(∆TM)-SspB plasmid has a deletion of transmembrane domain and is used for improved light inducible dimer assay.  

This resource can be used to generate knock-in cell line faciliating prime editing. 

This resource can be used to express and purify PE2 protein for RNP delivery.

This resource can be used to introduce HEK3 CTT insertion using prime editing. It serves as a good positive control for prime editing.

This resource can be used to introduce SNCA-A30P mutation using prime editing.

This resource can be used to introduce SNCA-A53T mutation using prime editing.

To make ATG9 C-terminal tail trimer for expression in mammalian cells.

The below plasmids are deposited and available via Addgene: https://www.addgene.org/Anthony_Schapira/. These have been used in publication: 10.1093/hmg/ddac233 188580 WT GBA pcDNA3.1 GBA (Homo sapiens) 188581 E326K GBA pcDNA3.1 GBA (Homo sapiens) 188582 L444P GBA pcDNA3.1 GBA (Homo sapiens) 188583 N370S GBA pcDNA3.1 GBA (Homo sapiens)

Tet-off TauRD (P301L/V337M). Associated with the following preprint: Saha et al., 2022 (published on biorxiv on February 19, 2022). https://www.biorxiv.org/content/10.1101/2022.02.18.481043v1.full

Associated with the following preprint: Saha et al., 2022 (published on biorxiv on February 19, 2022). https://www.biorxiv.org/content/10.1101/2022.02.18.481043v1.full

Tet-off Full Length Tau (0N4R, P301L/V337M). Associated with the following preprint: Saha et al., 2022 (published on biorxiv on February 19, 2022). https://www.biorxiv.org/content/10.1101/2022.02.18.481043v1.full

Associated with the following preprint: Saha et al., 2022 (published on biorxiv on February 19, 2022). https://www.biorxiv.org/content/10.1101/2022.02.18.481043v1.full

Expresses human ATG9-D830A/E831A mutant in mammalian cells.

Plasmid for bacterial expression of human PPM1H (H153D); contains TEV protease site for removal of 6His tag.

Plasmid for bacterial expression of human PPM1H (D288A); contains TEV protease site for removal of 6His tag.

This resource can be used to introduce HEK3 CTT insertion using prime editing, PE3 approach. It serves as a good positive control for prime editing.

Plasmid for the expression and purification of GST-TEV-NDP52 in E. coli Rosetta pLysS. Internal reference: SMC1586

This resource can be used to introduce LRRK2-G2019S mutation using CRISPR/Cas9 based genome editing.