pBMN_HA-TBK1 (kinase dead – D135N)
By savannah onUsed for the expression of TBK1 carrying a kinase dead mutation D135N (SMC Internal No. 1997).
pBMN_HA-TBK1 (E696K)
By savannah onUsed for the expression of TBK1 carrying E696K mutation (SMC Internal No. 1998).
HeLa S3 ATG3 KO
By savannah onHeLa S3 ATG3 KO, CVCL_C7IT Population: African American Knockout cell: Method=CRISPR/Cas9; HGNC; 20962; ATG3 Transformant: NCBI_TaxID; 333761; Human papillomavirus type 18 (HPV18) Miscellaneous: Cell line information from personal communication of Skulsuppaisarn M. Derived from sampling site: Uterus; cervix
cDNA clones for expression of MitoTag (OMP25) in mammalian cells
By savannah onPlasmids for expression of OMP25 (MitoTag) in human and mouse cells.
cDNA clones for expression of LysoTag (TMEM192) in mammalian cells
By savannah onPlasmids for expression of TMEM192 (LysoTag) in human and mouse cells.
cDNA clones for expression of GolgiTag (TMEM115) in mammalian cells
By savannah onPlasmids for expression of TMEM115 (GolgiTag) in human and mouse cells.
mCh-Miro1(∆TM)-SspB
By savannah onmCh-Miro1(∆TM)-SspB plasmid has a deletion of transmembrane domain and is used for improved light inducible dimer assay.
AAVS1-SA-neo-CAGGS-PE2-2A-GFP
By savannah onThis resource can be used to generate knock-in cell line faciliating prime editing.
pET30a(+)-PE2
By savannah onThis resource can be used to express and purify PE2 protein for RNP delivery.
pU6-pegRNA-HEK3-CTTins-px330-scaffold
By savannah onThis resource can be used to introduce HEK3 CTT insertion using prime editing. It serves as a good positive control for prime editing.
pU6-pegRNA-SNCA-A30P
By savannah onThis resource can be used to introduce SNCA-A30P mutation using prime editing.
pU6-pegRNA-SNCA-A53T
By savannah onThis resource can be used to introduce SNCA-A53T mutation using prime editing.
pCAG-MBP-Foldon-ATG9 (801-839)
By savannah onTo make ATG9 C-terminal tail trimer for expression in mammalian cells.
x4 GBA plasmids
By savannah onThe below plasmids are deposited and available via Addgene: https://www.addgene.org/Anthony_Schapira/. These have been used in publication: 10.1093/hmg/ddac233 188580 WT GBA pcDNA3.1 GBA (Homo sapiens) 188581 E326K GBA pcDNA3.1 GBA (Homo sapiens) 188582 L444P GBA pcDNA3.1 GBA (Homo sapiens) 188583 N370S GBA pcDNA3.1 GBA (Homo sapiens)