Lab Resource Archive

Mammalian expression of H153D mutation in PPM1H.

For expression of the mammalian NDP52 tagged with HA.

Used for the expression of TBK1 (SMC Internal No. 1996).

Used for the expression of TBK1 carrying a kinase dead mutation D135N (SMC Internal No. 1997).

Used for the expression of TBK1 carrying E696K mutation (SMC Internal No. 1998).

HeLa S3 ATG3 KO, CVCL_C7IT Population: African American Knockout cell: Method=CRISPR/Cas9; HGNC; 20962; ATG3 Transformant: NCBI_TaxID; 333761; Human papillomavirus type 18 (HPV18) Miscellaneous: Cell line information from personal communication of Skulsuppaisarn M. Derived from sampling site: Uterus; cervix

VPS13D mutated plasmid.

Plasmid.

VPS13D with deleted DHPH domain.

Plasmids for expression of OMP25 (MitoTag) in human and mouse cells.

Plasmids for expression of TMEM192 (LysoTag) in human and mouse cells.

Plasmids for expression of TMEM115 (GolgiTag) in human and mouse cells.

mCh-Miro1(∆TM)-SspB plasmid has a deletion of transmembrane domain and is used for improved light inducible dimer assay.  

This resource can be used to generate knock-in cell line faciliating prime editing. 

This resource can be used to express and purify PE2 protein for RNP delivery.

This resource can be used to introduce HEK3 CTT insertion using prime editing. It serves as a good positive control for prime editing.

This resource can be used to introduce SNCA-A30P mutation using prime editing.

This resource can be used to introduce SNCA-A53T mutation using prime editing.

To make ATG9 C-terminal tail trimer for expression in mammalian cells.

The below plasmids are deposited and available via Addgene: https://www.addgene.org/Anthony_Schapira/. These have been used in publication: 10.1093/hmg/ddac233 188580 WT GBA pcDNA3.1 GBA (Homo sapiens) 188581 E326K GBA pcDNA3.1 GBA (Homo sapiens) 188582 L444P GBA pcDNA3.1 GBA (Homo sapiens) 188583 N370S GBA pcDNA3.1 GBA (Homo sapiens)