Lab Resource Archive

Mammalian expression of ATG13 C-terminal and ULK1 MIT domain fusion complex.

Mammalian expression of ULK1 (836-1050) with MBP tag.

Mammalian expression of VPS15 with TwinStrep-Flag tag.

Mammalian expression of VPS34.

Human p62 with silent mutation for sip62 #5 (Thermo Scientific). Internal reference: SMC516 Plasmid for the expression of GFP labelled NEMO (SMC Internal No. 1977).

Human KAT8 gene with N-terminal V5 tag cloned into the pLV plasmid backbone under a EF1a promoter and including IRES puromycin selection cassette by Vectorbuilder.

Plasmid vector encoding a non-targeting sgRNA sequence under a U6 promoter and a mApple fluorescent reporter. Generated by Vectorbuilder in the pLV backbone

Plasmid vector encoding a sgRNA sequence which targets the human PINK1 promoter for CRISPRi, under a U6 promoter and a mApple fluorescent reporter. Generated by Vectorbuilder in the pLV backbone.

Plasmid vector encoding a sgRNA sequence which targets the human KAT8 promoter for CRISPRi, under a U6 promoter and a mApple fluorescent reporter. Generated by Vectorbuilder in the pLV backbone.

Plasmid vector encoding a sgRNA sequence which targets the KANSL1 promoter from CRISPRi, under a U6 promoter and a mApple fluorescent reporter. Generated by Vectorbuilder in the pLV backbone.

Plasmid for expression of the CAP-Mac AAV variant capsid.

Express VPS13C truncation mutant in mammalian cells (VPS13C^halo∆1235-1748).

Plasmid for the expression and purification of GST-TEV-EGFP-TBK1 in Spodoptera frugiperda cells (Sf9). Internal reference: SMC1631. Used for the expression and purification of mCherry labelled TBK1 (SMC Internal No.: 1675).

Mammalian expression of fluorescently-tagged marker for autophagic vesicles.

Mammalian expression of Q67L mutation in ARF6.

Mammalian expression of CFP.

Mammalian expression of fluorescently-tagged marker for mitochondria.

Mammalian expression of fluorescently-tagged marker for autophagic vesicles.

Mammalian expression of fluorescently-tagged marker for autophagic vesicles.

Mammalian expression of wild-type PPM1H.