pCAG-TSF-ATG13 (450-517)-ULK1 (836-1050)
By savannah onMammalian expression of ATG13 C-terminal and ULK1 MIT domain fusion complex.
pGEX-4T1-GST-Thrombin-TEV-EGFP-hNEMO
By savannah onHuman p62 with silent mutation for sip62 #5 (Thermo Scientific). Internal reference: SMC516 Plasmid for the expression of GFP labelled NEMO (SMC Internal No. 1977).
Plasmid: pLV[Exp]-EF1A>V5/hKAT8[NM_032188.3]:IRES:Puro
By savannah onHuman KAT8 gene with N-terminal V5 tag cloned into the pLV plasmid backbone under a EF1a promoter and including IRES puromycin selection cassette by Vectorbuilder.
Lentivirus plasmids for sgRNA: pLV[Exp]-U6>NT-Seq1-hPGK>mApple
By savannah onPlasmid vector encoding a non-targeting sgRNA sequence under a U6 promoter and a mApple fluorescent reporter. Generated by Vectorbuilder in the pLV backbone
“Plasmid vector encoding a sgRNA sequence which targets the human KAT8 promoter for CRISPRi, under a U6 promoter and a mApple fluorescent reporter. Generated by Vectorbuilder in the pLV[Exp] backbone.”
By savannah onPlasmid vector encoding a sgRNA sequence which targets the human PINK1 promoter for CRISPRi, under a U6 promoter and a mApple fluorescent reporter. Generated by Vectorbuilder in the pLV backbone.
Lentivirus plasmid: pLV[Exp]-U6>KAT8_P1_Seq1-hPGK>mApple
By savannah onPlasmid vector encoding a sgRNA sequence which targets the human KAT8 promoter for CRISPRi, under a U6 promoter and a mApple fluorescent reporter. Generated by Vectorbuilder in the pLV backbone.
Lentivirus plasmid: pLV[Exp]-U6>KANSL1_P1_Seq1-hPGK>mApple
By savannah onPlasmid vector encoding a sgRNA sequence which targets the KANSL1 promoter from CRISPRi, under a U6 promoter and a mApple fluorescent reporter. Generated by Vectorbuilder in the pLV backbone.
VPS13C^halo∆1235-1748
By savannah onExpress VPS13C truncation mutant in mammalian cells (VPS13C^halo∆1235-1748).
pFastBac_Dual_GST-TEV-mCherry-TBK1
By savannah onPlasmid for the expression and purification of GST-TEV-EGFP-TBK1 in Spodoptera frugiperda cells (Sf9). Internal reference: SMC1631. Used for the expression and purification of mCherry labelled TBK1 (SMC Internal No.: 1675).
PGK EGFP-LC3B
By savannah onMammalian expression of fluorescently-tagged marker for autophagic vesicles.
PGK 4xMito-mEmerald
By savannah onMammalian expression of fluorescently-tagged marker for mitochondria.
CMV mScarlet-LC3B
By savannah onMammalian expression of fluorescently-tagged marker for autophagic vesicles.
PGK mScarlet-LC3B
By savannah onMammalian expression of fluorescently-tagged marker for autophagic vesicles.