pAAV_Ef1a_DIO_hTyrNull_HATag
By Julia Julia Leonard onCre-dependent expression of a truncated human tyrosinase is inactive and does not lead to the accumulation of neuromelanin in catecholaminergic neurons (control virus). Strong expression of Ef1a promoter, HA tag for histological verification of expression.
pAAV_Ef1a_DIO_hTyrHA_minBack
By Julia Julia Leonard onCre-dependent expression of human tyrosinase induces the formation of neuromelanin in catecholaminergic neurons. Expression under strong Ef1a promoter.
pAAV_Cbh_DIO_hTyr
By Julia Julia Leonard onCre-dependent expression of human tyrosinase induces the formation of neuromelanin in catecholaminergic neurons. The CbH promoter is a strong, pan-neuronal, non-silencable promoter.
pAAV_CbH_SIO_SyGCaMP8s_p2a_mRuby
By Julia Julia Leonard onThis construct links GCaMP8s to Synaptophysin and directs the fusion protein to presynaptic sites. It also produces a cytoplasmic red fluorescence protein (mRuby) via the self-splicing peptide 2A. It is mainly used to longitudinally image synaptic activity with 2-Photon at 980nm and visualize axons at 1080nm in vivo.
HEK293T SNCA-A53T-mRuby3 SNCA-A53T-mClover3
By Emma Sherrell onCell Line: Transfected with two plasmids encoding mRuby3- and mClover3-tagged SNCA p.Ala53Thr expressed under the control of CMV promoters.
HEK293E_Ecto short VLDLR-C-tag
By Emma Sherrell onCell Line: Transfected with the pB-T-PAF expression plasmid expressing the ectodomain of VLDLR fused to a C-terminal C-tag (EPEA) under the tet operator and the PB-RN reverse transactivator plasmid expressing the reverse tetracycline-controlled transactivator under the CMV promoter.
HEK293E_Clu WT
By Emma Sherrell onCell Line: Transfected with the pB-T-PAF expression plasmid expressing CLU under the tet operator and the PB-RN reverse transactivator plasmid expressing the reverse tetracycline-controlled transactivator under the CMV promoter.
HEK293E_Clu TL4
By Emma Sherrell onCell Line: Transfected with the pB-T-PAF expression plasmid expressing CLU under the tet operator and the PB-RN reverse transactivator plasmid expressing the reverse tetracycline-controlled transactivator under the CMV promoter.
HEK293E_Clu TL3
By Emma Sherrell onCell Line: Transfected with the pB-T-PAF expression plasmid expressing CLU under the tet operator and the PB-RN reverse transactivator plasmid expressing the reverse tetracycline-controlled transactivator under the CMV promoter.
HEK293E_Clu TL2+3
By Emma Sherrell onCell Line: Transfected with the pB-T-PAF expression plasmid expressing CLU under the tet operator and the PB-RN reverse transactivator plasmid expressing the reverse tetracycline-controlled transactivator under the CMV promoter.
HEK293E_Clu TL1
By Emma Sherrell onCell Line: Transfected with the pB-T-PAF expression plasmid expressing CLU under the tet operator and the PB-RN reverse transactivator plasmid expressing the reverse tetracycline-controlled transactivator under the CMV promoter.
HEK293E_Clu DD3
By Emma Sherrell onCell Line: Transfected with the pB-T-PAF expression plasmid expressing CLU under the tet operator and the PB-RN reverse transactivator plasmid expressing the reverse tetracycline-controlled transactivator under the CMV promoter.
HEK293E_Clu DD1
By Emma Sherrell onCell Line: Transfected with the pB-T-PAF expression plasmid expressing CLU under the tet operator and the PB-RN reverse transactivator plasmid expressing the reverse tetracycline-controlled transactivator under the CMV promoter.
HEK293E_Clu CC3
By Emma Sherrell onCell Line: Transfected with the pB-T-PAF expression plasmid expressing CLU under the tet operator and the PB-RN reverse transactivator plasmid expressing the reverse tetracycline-controlled transactivator under the CMV promoter.
HEK293E_Clu CC2
By Emma Sherrell onCell Line: Transfected with the pB-T-PAF expression plasmid expressing CLU under the tet operator and the PB-RN reverse transactivator plasmid expressing the reverse tetracycline-controlled transactivator under the CMV promoter.
HEK293E_Clu CC1
By Emma Sherrell onCell Line: Transfected with the pB-T-PAF expression plasmid expressing CLU under the tet operator and the PB-RN reverse transactivator plasmid expressing the reverse tetracycline-controlled transactivator under the CMV promoter.
HEK293E_Clu AB2
By Emma Sherrell onCell Line: Transfected with the pB-T-PAF expression plasmid expressing mutated CLU under the tet operator and the PB-RN reverse transactivator plasmid expressing the reverse tetracycline-controlled transactivator under the CMV promoter.
pLenti HsATP13A3 D498N
By Julia Julia Leonard onPlasmid: Transfer plasmid for lenti viral vector production, expresses D498N mutant of Homo sapiens ATP13A3.