Protocol Archive

Here, the authors present a protocol for quantitative immunostaining of intracellular accessible cholesterol using ALOD4-mNeon in human fibroblasts.

Here, the authors describe an algorithm for segmenting fluorescently-labeled cilia using SSTR3 as a ciliary marker.

Here, the authors describe a protocol for RNA extraction, cDNA synthesis, and quantitative PCR from NIH-3T3 fibroblasts. This method has also been used with mouse embryonic fibroblasts.

Here, the authors present a CellProfiler (PMID: 34507520) software pipeline to quantify the density and intensity of dopaminergic processes in the mouse striatum. The dopaminergic processes in the striatum are stained using an anti-tyrosine hydroxylase antibody and the same sections are also stained using an antibody that recognizes total neuronal NeuN (neuronal nuclear antigen biomarker) for staining normalization.

Culturing of iPSC lines in essential 8 medium, thawing, and passaging techniques.

This protocol describes the authors' method for the differentiation of human floor plate neural progenitor cells into human midbrain-dopaminergic neurons. This protocol has been developed using a combination of several published protocols.

The intent of this Standard Operating Procedure (SOP) is to describe procedures for mouse spinal cord injection surgery.

Protocol used in the Mazmanian lab for collecting brain and gut tissues and plasma from mouse for metabolomics.

Protocol for using the Biocrates MxP-Q500 kit (BIOCRATES Life Science AG, Innsbruck, Austria), a commercially-available targeted metabolomics platform, to measure 634 metabolites across 26 biochemical classes from different tissues of the mouse.

How to create simulated synapse images using FIJI.

This protocol described the production of human cortical neurons from induced pluripotent stem cells.

This protocol describes the capture of eGFP-L10a-tagged ribosomes and mRNA from DAT-expressing cells (dopaminergic neurons) in mouse ventral midbrain.

This protocol describes how to isolate crude mitochondrial fractions from HeLa cells.

Surgery and recording protocol for measuring sleep-wake behavior in mice for Turek Laboratory.

This protocol describes SDS-PAGE and Western blot analysis.

This protocol describes Rapalog-induced chemical dimerization experiments.

This protocol describes the purification of mCherry-OPTN.

This protocol describes how to induce mitophagy in HeLa cells.

This protocol describes how to induce bulk (non-selective) autophagy in HeLa cells through nutrient starvation.

This protocol describes purification of GST-NAP1 and unlabeled NAP1.