Intramuscular PFF injection
By Emma Sherrell onThis protocol details the intramuscular PFF injection procedure. This protocol details the intramuscular PFF injection procedure.
Synthesis of 1-(3-Chlorophenethyl)-3-cyclopentylpyrimidine-2,4,6-(1H,3H,5H)-trione
By Julia Julia Leonard on1-(3-chlorophenethyl)-3-cyclopentylpyrimidine-2,4,6-(1H,3H,5H)-trione (8) is a potent and highly selective Ca(V)1.3 L-type calcium channel antagonist. This protocol is an adaption of Kang et al., 2012's method of the synthesis of this chemical.
Open field
By Emma Sherrell onThis protocol details about Open Field procedure. This protocol details about Open Field procedure.
AAV injection in the nodose ganglia of mouse
By Emma Sherrell onThe study of these ganglia is challenging in small animals due to size and location. Hence, in this protocol, the team describes a practical surgical approach to the vagal trunk, and the JNG complex in mice.
Automatic flow in fluid-walled dumbbells driven by Laplace pressure
By Julia Julia Leonard onThis protocol describes experiments performed to quantify pressure and volume variations inside fluid-walled dumbbells when a pressure difference is generated between the two chambers. Difference in Laplace pressure automatically flows medium from the high-pressure chamber to the low pressure one. Flow stops when pressures are equilibrated.
Immunostaining of corticostriatal culture on fluid-walled dumbbells
By Julia Julia Leonard onThis protocol describes the immunocytochemistry of the iPSC-derived corticostriatal culture on fluid-walled dumbbells.
Fura-2 imaging of ionomycin response, with and without R568; a CaSR positive modulator
By Julia Julia Leonard onFura-2 imaging protocol to accompany Kilfeather, Khoo et al., 2023: Single cell spatial transcriptomic and translatomic profiling of dopaminergic neurons in health, aging, and disease.
Generation of stable cell lines using retroviral system
By Emma Sherrell onThis protocol details generation of stable cell lines using retroviral system.
Immunocytochemistry for CASR in iPSc-derived dopaminergic neurons
By Julia Julia Leonard onCASR immunocytochemistry protocol to accompany Kilfeather, Khoo et al., 2023: Single cell spatial transcriptomic and translatomic profiling of dopaminergic neurons in health, aging, and disease.
Assessing autophagy using the HaloTag-LC3B cleavage assay
By Emma Sherrell onAssay to detect HaloTag-LC3B during starvation autophagy based on https://doi.org/10.7554/eLife.78923.
DAT-TRAP Protocol
By Julia Julia Leonard onThis protocol describes the capture of eGFP-L10a-tagged ribosomes and mRNA from DAT-expressing cells in mouse ventral midbrain.
Fast-scan cyclic voltammetry to assess dopamine release in ex vivo mouse brain slices
By Julia Julia Leonard onThis protocol is to assess whether a drug changes the dopamine concentration released following a single pulse (1p) electrical stimulation.
Whole mount dissection and staining of enteric nervous system
By Julia Julia Leonard onThis protocol details whole mount dissection and staining of the enteric nervous system.
Protocol for in vivo electrophysiological and optogenetic manipulation of basal ganglia neurons in awake head-fixed mice
By Julia Julia Leonard onStep by step procedure for in vivo electrophysiological and optogenetic manipulation of basal ganglia neurons in awake head-fixed mice.
Headplate surgery protocol for in vivo electrophysiological and optogenetic manipulation of basal ganglia neurons in awake head-fixed mice
By Julia Julia Leonard onStep by step protocol for headplate surgery for in vivo electrophysiological and optogenetic manipulation of basal ganglia neurons in awake mice.
Stereotaxic injection of viral vectors
By Julia Julia Leonard onStereotaxic injection of viral vectors.
Protocol for ex vivo patch clamping
By Julia Julia Leonard onProtocol for ex vivo patch clamping Protocol for ex vivo patch clamping.