Regional mouse brain analysis (Modified QUINT)
By Emma Sherrell onThis series of protocols has been adapted from published and unpublished protocols broadly referred to as the QUINT workflow: Note that the original QUINT workflow was generated by Yates and colleagues, and all credit for the development of these programs goes to that team.
Live cell imaging of yeast cells expressing human PINK1-GFP and the TOM complex subunits
By Emma Sherrell onA protocol for live-cell imaging of yeast cells expressing human PINK1 with GFP tag at the C-terminus as well as all the human TOM complex subunits (TOMs 5, 6, 7, 20, 22, 40 and 70).
Single-molecule antibody slides for fluorescence microscopy
By Emma Sherrell onThis protocol describes how to create single-molecule antibody slides for fluorescence microscopy.
Tetraspeck bead imaging V.2
By Emma Sherrell onProtocol for imaging tetraspeck beads on glass coverslips.
Rating scale for Parkinsonian motor signs in macaques and other non‐human primates
By Emma Sherrell onThis protocol details rating scale for Parkinsonian motor signs in macaques and other non‐human primates.
T cell differentiation from mice spleen tissue
By Emma Sherrell onThis protocol is for T cell differentiation from mice spleen tissue to investigate T cell function (including differentiation and proliferation) in vitro.
Genetic modification of human induced pluripotent stem cells (hiPSCs) by lentiviral transduction protocol
By Emma Sherrell onThis is the protocol for the genetic modification of human induced pluripotent stem cells (hiPSCs) using lentiviral transduction method.
Cytotoxicity Assay Protocol
By Emma Sherrell onThis is the protocol for cytotoxicity assay in cancer cell lines using flow cytometry.
Preparation of Holding ACSF (artificial cerebrospinal fluid) for slice electrophysiology
By Emma Sherrell onHolding ACSF is used for incubating brain slices before cell-attached or whole-cell electrophysiological recordings of neurons.
Preparation of Recording ACSF (artificial cerebrospinal fluid) for slice electrophysiology
By Emma Sherrell onRecording ACSF is used for incubating brain slices during cell-attached or whole-cell electrophysiological recordings of neurons.
v2 RNAscope
By Emma Sherrell onThis is a protocol for performing RNAscope® in situ hybridization analysis on fixed-frozen mouse brain tissue using the RNAscope® Multiplex Fluorescent v2 kit (Advanced Cell Diagnostics; ACD).
Imaging and analysis of mouse midbrain tissue labeled by RNAscope
By Emma Sherrell onThe authors describe imaging, analysis, and cell counts of coronal sections of mouse substantia nigra and ventral tegmental area labeled with Slc17a6 (VGLUT2), Slc32a1 (VGAT), and Slc18a2 (VMAT2).
Preparation and RNAscope-labeling of fresh mouse midbrain tissue
By Emma Sherrell onThe authors describe preparing and labeling coronal sections of mouse substantia nigra (SNc) and ventral tegmental area (VTA) for probes targeting the genes Slc17a6 (VGLUT2), Slc32a1 (VGAT), and Slc18a2 (VMAT2).
LC/MS analysis of plasma samples from PPMI
By Emma Sherrell onPlasma samples from PPMI were analyzed by liquid chromatography with mass spectrometry (LC/MS) for a variety of metabolites (including piperine) and lipids as interrelated markers of Parkinson's disease and its pathophysiology.
Purification of total RNA from cells using spin technology
By Emma Sherrell onPurification of total RNA from cells using spin technology.
Analysis of glycosphingolipids from animal tissues
By Emma Sherrell onInterest in the role of cellular glycosphingolipids (GSLs) in health and disease led the authors to develop a sensitive method to analyze the full complement of GSL structures present in mammalian cells, fluids, and tissues.
In-vivo MRI and CT scanning protocol for non-human primates using a 3D-printed MR-compatible stereotaxic frame
By Emma Sherrell onThis protocol describes the methods of using a custom 3D printed stereotaxic frame to acquire MRI and CT scans for nonhuman primates in detailed steps.
DAB staining for GFP on free-floating fixed NHP brain tissue
By Emma Sherrell onThis protocol details the procedure for immunohistochemical 3,3’-Diaminobenzidine (DAB) staining of free-floating fixed brain tissue sections using the avidin/biotin ABC complex to detect green fluorescent protein (GFP).
Standard DAB staining for free-floating fixed NHP brain tissue
By Emma Sherrell onThis protocol details the procedure for immunohistochemical 3,3’-Diaminobenzidine (DAB) staining of free-floating fixed brain tissue sections using the avidin/biotin ABC complex.
Free-floating immunofluorescence protocol on mouse brain sections for tau pathology
By Emma Sherrell onThis protocol describes the free-floating multiplexed immunofluorescent staining protocol to ascertain levels of tau and phospho-tau in mouse tissue from transplanted human iPSC cells carrying different PD-related mutations.