Protocol Particulars

The ASAP-sponsored interview series, Protocol Particulars, highlights ASAP-generated protocols. Featured guests include the protocol authors, who provide an overview of their protocol, describe how the protocol relates to their ASAP project, and present detailed troubleshooting tips. 

Protocol Particulars is designed to further the spread of knowledge and enhance equity across the scientific community through promoting transparent research practices and enabling others to replicate the work. We are excited to share these interviews with the research community, in hopes that they will help to accelerate discoveries for Parkinson’s disease. 

Watch Protocol Particulars

Watch the videos or listen in audio-only mode. To receive notifications when there are new interviews, subscribe to ASAP’s YouTube channel.

Verifying the Integrity of Data Sets Uploaded to Amazon S3 with Sonia García-Ruiz, PhD

Sonia García-Ruiz, a postdoctoral fellow on Team Hardy, chatted with ASAP about the challenges associated with generating and storing large biomedical data sets and a new tool that enables users to determine if any unwanted changes occurred when uploading a dataset to Amazon S3. 

Read the full protocol. Learn more about Team Hardy. Watch the full video or listen to the audio transcript to learn more. 

Uncovering Mitochondrial Antigen Presentation with Ahmed Fahmy, PhD

Sonia García-Ruiz, a postdoctoral fellow on Team Hardy, chatted with ASAP about the challenges associated with generating and storing large biomedical data sets and a new tool that enables users to determine if any unwanted changes occurred when uploading a dataset to Amazon S3. 

Read the full protocol. Learn more about Team Hardy.

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Uncovering Mitochondrial Antigen Presentation with Ahmed Fahmy, PhD

In this episode of Protocol Particulars, ASAP speaks with Ahmed Fahmy, PhD from Team Desjardins to learn more about best practices when conducting mitochondrial antigen presentation assays.

Read the full protocol. Learn more about Team DesjardinsWatch the full video or listen to the audio transcript to learn more.

Uncovering Mitochondrial Antigen Presentation with Ahmed Fahmy, PhD

In this episode of Protocol Particulars, ASAP speaks with Ahmed Fahmy, PhD from Team Desjardins to learn more about best practices when conducting mitochondrial antigen presentation assays.

Read the full protocol. Learn more about Team Desjardins. Watch the full video or listen to the audio transcript to learn more.

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Assessing Cellular Uptake of Fluorescently-labeled Polyamines with Mujahid Azfar, MSc

In this episode, Protocol Particulars host, Devin Snyder, chats with Mujahid Azfar, MSc, a graduate student at KU Leuven and member of Team Vangheluwe about the protocol, “Fluorescently-labeled Polyamine Uptake (via Flow Cytometry),” which is authored by Team Vangheluwe alum Marine Houdou. 

Read the full protocol. Learn more about Team Vangheluwe. Watch the full video or listen to the audio transcript to learn more.

Assessing Cellular Uptake of Fluorescently-labeled Polyamines with Mujahid Azfar, MS

In this episode, Protocol Particulars host, Devin Snyder, chats with Mujahid Azfar, MSc, a graduate student at KU Leuven and member of Team Vangheluwe about the protocol, “Fluorescently-labeled Polyamine Uptake (via Flow Cytometry),” which is authored by Team Vangheluwe alum Marine Houdou. 

Read the full protocol. Learn more about Team Vangheluwe. Watch the full video or listen to the audio transcript to learn more.

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Free-floating Mouse Brain Immunohistochemistry with Jonathan Breiter, MSc

Jonathan Breiter, MSc, joins ASAP’s Protocols Particulars in a discussion on the protocol, “Free-floating Mouse Brain Immunohistochemistry.” Here, Jonathan provides insight into the pros and cons of free-floating vs. slide-mounted tissue staining and gives a few tricks for ensuring that the tissue remains intact.

Read the full protocol. Learn more about Team Wood. Watch the full video or listen to the audio transcript to learn more.

Free-floating immunohistochemistry with Jonathan Breiter, MSc

Jonathan Breiter, MSc, joins ASAP’s Protocols Particulars in a discussion on the protocol, “Free-floating Mouse Brain Immunohistochemistry.” Here, Jonathan provides insight into the pros and cons of free-floating vs. slide-mounted tissue staining and gives a few tricks for ensuring that the tissue remains intact.

Read the full protocol. Learn more about Team Wood. Watch the full video or listen to the audio transcript to learn more.

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Rapid Purification of Intact Golgi with Rotimi Fasimoye, PhD

On this episode of Protocol Particulars, ASAP staff are joined by Team Alessi member, Rotimi Fasimoye, PhD. Here, Rotimi describes the ins and outs of his protocol, “Introducing GolgiTag to Cells and Immunoprecipitation of Golgi.”

Read the full protocol. Learn more about Team Alessi. Watch the full video or listen to the audio transcript to learn more.

Rapid Purification of Intact Golgi with Rotimi Fasimoye, PhD

On this episode of Protocol Particulars, ASAP staff are joined by Team Alessi member, Rotimi Fasimoye, PhD. Here, Rotimi describes the ins and outs of his protocol, “Introducing GolgiTag to Cells and Immunoprecipitation of Golgi.”

Read the full protocol. Learn more about Team Alessi. Watch the full video or listen to the audio transcript to learn more.

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iNeuron Differentiation on EM Grids with Cristina Capitanio, MSc

Cristina Capitanio, MSc, a graduate student at the Max Planck Institute of Biochemistry and member of Team Harper, joins ASAP’s Protocol Particulars to share her protocol on neural differentiation on EM grids – iNeurons Sample Preparation for Cryo-ET and CLEM. 

Read the full protocol. Learn more about Team HarperWatch the full video or listen to the audio transcript to learn more. 

iNeuron differentiation on EM grids with Cristina Capitanio, MSc

Cristina Capitanio, MSc, a graduate student at the Max Planck Institute of Biochemistry and member of Team Harper, joins ASAP’s Protocol Particulars to share her protocol on neural differentiation on EM grids – iNeurons sample preparation for cryo-ET and CLEM. 

Read the full protocol. Learn more about Team HarperWatch the full video or listen to the audio transcript to learn more. 

Play Video about ASAP Protocol Particulars iNeuron Differentiation CristinaC_

Immunoblotting Methods for LRRK2 Signaling with Francesca Tonelli, PhD

On this episode of Protocol Particulars, Francesa Tonelli, PhD, a senior research scientist at the University of Dundee and project manager for Team Alessi, joins ASAP to discuss her protocol for the quantitative immunoblotting analysis of the LRRK2 signaling pathway. 

Read the full protocol. Learn more about Team Alessi. Watch the full video or listen to the audio transcript to learn more. 

Immunoblotting Methods for LRRK2 Signaling with Francesca Tonelli, PhD

On this episode of Protocol Particulars, Francesa Tonelli, PhD, a senior research scientist at the University of Dundee and project manager for Team Alessi, joins ASAP to discuss her protocol for the quantitative immunoblotting analysis of the LRRK2 signaling pathway. 

Read the full protocol. Learn more about Team Alessi. Watch the full video or listen to the audio transcript to learn more. 

Play Video about ASAP Protocol Particulars Immunoblotting MethodsFrancescaT_1280x720_v1r1

Examining the Ultrastructural Components of Dopamine Neurons with Natalie Doig, PhD

Natalie Doig, PhD, a postdoc at the University of Oxford and member of Team Cragg, joins ASAP’s Protocol Particulars to discuss her collection of protocols on the quantitative analysis of ultrastructural features of dopaminergic axon terminals.

Read the full protocol. Learn more about Team CraggWatch the full video or listen to the audio transcript to learn more. 

Examining the Ultrastructural Components of Dopamine Neurons with Natalie Doig, PhD

Natalie Doig, PhD, a postdoc at the University of Oxford and member of Team Cragg, joins ASAP’s Protocol Particulars to discuss her collection of protocols on the quantitative analysis of ultrastructural features of dopaminergic axon terminals.

Read the full protocol. Learn more about Team CraggWatch the full video or listen to the audio transcript to learn more. 

Play Video about ASAP Protocol Particulars Examining the Ultrastructural ComponentsNatalie D

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