Protocol Particulars

The ASAP-sponsored interview series, Protocol Particulars, highlights ASAP-generated protocols. Featured guests include the protocol authors, who provide an overview of their protocol, describe how the protocol relates to their ASAP project, and present detailed troubleshooting tips. 

Protocol Particulars is designed to further the spread of knowledge and enhance equity across the scientific community through promoting transparent research practices and enabling others to replicate the work. We are excited to share these interviews with the research community, in hopes that they will help to accelerate discoveries for Parkinson’s disease. 

Watch Protocol Particulars

Watch the videos or listen in audio-only mode. To receive notifications when there are new interviews, subscribe to ASAP’s YouTube channel.

Examining the Ultrastructural Components of Dopamine Neurons with Natalie Doig, PhD

Natalie Doig, PhD, a postdoc at the University of Oxford and member of Team Cragg, joins ASAP’s Protocol Particulars to discuss her collection of protocols on the quantitative analysis of ultrastructural features of dopaminergic axon terminals.

Read the full protocol. Learn more about Team CraggWatch the full video or listen to the audio transcript to learn more. 

Examining the Ultrastructural Components of Dopamine Neurons with Natalie Doig, PhD

Natalie Doig, PhD, a postdoc at the University of Oxford and member of Team Cragg, joins ASAP’s Protocol Particulars to discuss her collection of protocols on the quantitative analysis of ultrastructural features of dopaminergic axon terminals.

Read the full protocol. Learn more about Team CraggWatch the full video or listen to the audio transcript to learn more. 

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Immunoblotting Methods for LRRK2 Signaling with Francesca Tonelli, PhD

On this episode of Protocol Particulars, Francesa Tonelli, PhD, a senior research scientist at the University of Dundee and project manager for Team Alessi, joins ASAP to discuss her protocol for the quantitative immunoblotting analysis of the LRRK2 signaling pathway. 

Read the full protocol. Learn more about Team Alessi. Watch the full video or listen to the audio transcript to learn more. 

Immunoblotting Methods for LRRK2 Signaling with Francesca Tonelli, PhD

On this episode of Protocol Particulars, Francesa Tonelli, PhD, a senior research scientist at the University of Dundee and project manager for Team Alessi, joins ASAP to discuss her protocol for the quantitative immunoblotting analysis of the LRRK2 signaling pathway. 

Read the full protocol. Learn more about Team Alessi. Watch the full video or listen to the audio transcript to learn more. 

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iNeuron differentiation on EM grids with Cristina Capitanio, MSc

Cristina Capitanio, MSc, a graduate student at the Max Planck Institute of Biochemistry and member of Team Harper, joins ASAP’s Protocol Particulars to share her protocol on neural differentiation on EM grids – iNeurons sample preparation for cryo-ET and CLEM. 

Read the full protocol. Learn more about Team HarperWatch the full video or listen to the audio transcript to learn more. 

iNeuron differentiation on EM grids with Cristina Capitanio, MSc

Cristina Capitanio, MSc, a graduate student at the Max Planck Institute of Biochemistry and member of Team Harper, joins ASAP’s Protocol Particulars to share her protocol on neural differentiation on EM grids – iNeurons sample preparation for cryo-ET and CLEM. 

Read the full protocol. Learn more about Team HarperWatch the full video or listen to the audio transcript to learn more. 

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