Mito-Keima assay to assess mitophagy

By Thanh Nguyen, PhD
View Published Protocol

Output Details

Description
This protocol details the procedure of mito-keima assay to assess mitophagy.
Identifier (DOI)
10.17504/protocols.io.q26g74e1qgwz/v1
Tags
  • Other
Team
Team Hurley
Program
Collaborative Research Network
Theme
PD Functional Genomics

Meet the Authors

  • Thanh Nguyen, PhD

    Key Personnel: Team Hurley, 

    Walter and Eliza Hall Institute of Medical Research

Related Research

Unconventional Initiation of PINK1/Parkin Mitophagy by Optineurin

This work reveals that OPTN mitophagy initiation is mechanistically distinct and highlights the mechanistic plasticity of selective autophagy pathways.

View Article

Control of mitophagy initiation and progression by the TBK1 adaptors NAP1 and SINTBAD

Mitophagy maintains mitochondrial health by degrading damaged mitochondria. TBK1 adaptors NAP1 and SINTBAD regulate OPTN-driven mitophagy initiation and NDP52-driven progression, balancing pathway activity for efficient functioning.

View Article

Reconstitution of BNIP3/NIX-mediated autophagy reveals two pathways and hierarchical flexibility of the initiation machinery

During selective autophagy transmembrane cargo receptors can trigger autophagy by recruiting different complexes and utilizing multiple pathways. This flexibility in autophagy initiation has important therapeutic implications.

View Article
View More Outputs