This website uses cookies so that we can provide you with the best user experience possible. Cookie information is stored in your browser and performs functions such as recognising you when you return to our website and helping our team to understand which sections of the website you find most interesting and useful.
Staining of cells with GolgiTracker for Golgi flow cytometry analysis
Output Details
Description
We describe a method that allows the staining of intact Golgi using GolgiTracker for subsequent flow cytometry analysis. The analysis can provide a quantitative indication of the level of enrichment of the organelle immunoprecipitation (% of GOLGI+ beads) and can be used as a quality control check for the purity of the immunoprecipitated Golgi.
Identifier (DOI)
10.17504/protocols.io.e6nvwk1d2vmk/v1