Golgi-IP, a novel tool for multimodal analysis of Golgi molecular content
Published May 8, 2023
Output Details
Preprint November 23, 2022
Published May 8, 2023
Description
The Golgi is central to protein and lipid processing. It senses and responds to diverse cell states to allow trafficking of macromolecules based on cellular demands. Traditional techniques for purifying the Golgi shaped our understanding of its functions; however, such methods are too slow to preserve the labile Golgi metabolome and transient protein interactions. Here, we overcome this issue through the development of a method for the rapid capture of intact Golgi from human cells via Golgi immunoprecipitation (Golgi-IP). Using high-resolution mass spectrometry, we demonstrate that our approach allows the unbiased characterization of the Golgi proteome, metabolome, and lipidome. We believe that the Golgi-IP will be useful for the study of the Golgi in health and disease states.
Identifier (DOI)
10.1073/pnas.2219953120
