Preparation and imaging of enriched Golgi from GolgiTAG-IP using Transmission Electron Microscopy

By Rotimi Fasimoye, PhD and Alan Prescott
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Output Details

Description

Transmission electron microscopy (TEM) is a tool used to image, in good resolution, the structure of organelles within the cell. Available protocols are designed to image structures within fixed intact cells. Here, we described a protocol where Golgi, isolated from cells by GolgiTAG immunoprecipitation (IP) (as described in ddx.doi.org/10.17504/protocols.io.6qpvrdjrogmk/v1), can be prepared and imaged using TEM. This protocol can also be used to image any organelles isolated using various organelle-IP protocols that are available.

Identifier (DOI)
10.17504/protocols.io.x54v9y9nqg3e/v1
Tags
  • Electron microscopy
  • Golgi apparatus
  • Immunoprecipitation
Team
Team Alessi
Program
Collaborative Research Network
Theme
PD Functional Genomics

Meet the Authors

  • Rotimi Fasimoye, PhD

    Key Personnel: Team Alessi, 

    University of Dundee

  • User avatar fallback logo

    Alan Prescott

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