Formalin Fixed Paraffin Embedded (FFPE) Tissue Preparation for Digital Pathology Quantification Using QuPath

Description

QuPath is an open-source digital pathology analysis software. Here, it is used to quantify various misfolded proteins across anatomical regions. This protocol is appropriate for both human and mouse tissue, and will outline the methods of tissue preparation, digitisation, segmentation and importing images into QuPath.

Identifier (DOI)

10.17504/protocols.io.bp2l6x4jklqe/v1

Tags

Digital pathology
Formalin-fixed paraffin-embedded
Post-mortem brain tissues
Quantification
QuPath

Team

Team Hardy

Program

Collaborative Research Network

Theme

PD Functional Genomics

Meet the Authors

Zane Jaunmuktane, MD

Co-PI (Core Leadership): Team Hardy, Team Voet, Team Wood,

University College London
Hemanth Ramesh Nelvagal, PhD

Key Personnel: Team Hardy,

University College London
Toby Curless, MSc

Key Personnel: Team Hardy,

University College London

Formalin Fixed Paraffin Embedded (FFPE) Tissue Preparation for Digital Pathology Quantification Using QuPath

Output Details

Meet the Authors

Zane Jaunmuktane, MD

Hemanth Ramesh Nelvagal, PhD

Toby Curless, MSc

Related Research

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