CRISPR/Cas9 generation of knock-out iPSCs

Description

This protocol describes RNP-based CRISPR/Cas9 gene-editing to generate knock-out iPSCs. iPSCs are transfected with sgRNA, recombinant Cas9, and GFP using Lipofectamine Stem. After FACS sorting to enrich for successfully transfected cells, individual clones are picked and expanded for further analysis.

Identifier (DOI)

10.17504/protocols.io.36wgqj48yvk5/v1

Tags

Cas9
CRISPR
iPSCs (Induced pluripotent stem cells)

Team

Team Hurley

Program

Collaborative Research Network

Theme

PD Functional Genomics

Meet the Authors

Dan Dou

Key Personnel: Team Hurley,

University of Pennsylvania
Alexander Boecker, MD

Key Personnel: Team Hurley,

University of Pennsylvania
Erika Holzbaur, PhD

Co-PI (Core Leadership): Team Hurley,

University of Pennsylvania

CRISPR/Cas9 generation of knock-out iPSCs

Output Details

Meet the Authors

Dan Dou

Alexander Boecker, MD

Erika Holzbaur, PhD

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