Drd1-T2A-FlpO Mouse Line

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Output Details

Description
Dopamine D1 receptor FlpO mouse line generated by the Costa lab at Cyagen using CRISPR/Cas-mediated genome engineering. For this model the TGA stop codon in exon 2 of mouse Drd1 gene (NCBI Reference Sequence: NM_001291801.1) was identified and this stop codon was replaced with "T2A-Flpo"cassette. Cas9 and gRNA was co-injected into fertilized eggs with the donor vector for mouse production.
Tags
  • Dopamine
  • Mouse
  • Mouse model
Team
Team Surmeier
Program
Collaborative Research Network
Theme
Circuitry and Brain-Body Interactions

Related Research

Generation of knock-in Cre and FlpO mouse lines for precise targeting of striatal projection neurons and dopaminergic neurons

To enable precise genetic targeting of key neuronal populations in the basal ganglia, we generated and characterized five knock-in mouse lines: Drd1-Cre, Adora2a-Cre, Drd1-FlpO, Adora2a-FlpO, and DAT-FlpO.

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Drd1-T2A-Cre mouse line

Dopamine D1 receptor cre knock in mouse line generated by the Costa lab.

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Slc6a3-T2A-FlpO (DAT-flpO) Mouse Line

DAT-FlpO knock-in mice express optimized Flp recombinase (FlpO) from the mouse Slc6a3 (DAT) promoter in Slc6a3-expressing cells, including dopaminergic neurons. This strain is useful in studies of motor neuron deficits in Parkinson's disease.

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Aligning Science Across Parkinson's
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