Slc6a3-T2A-FlpO (DAT-flpO) Mouse Line

To generate DAT-FlpO mice, a gRNA (5’ CCAACAGCCAATGGCGCAGC-TGG 3’) targeting exon 15, and a template with a ‘2A-FlpO-rBG pA’ cassette were used. Homology arms were PCR amplified from BAC clones RP23-150M11 and RP23-34F24. Two silent mutations, aa 613L (CTG to TTA), and aa 614R (CGC to AGG) were introduced to prevent re-cleavage by Cas9 after homology-directed repair.