Immunohistochemistry
By onThis protocol describes immunohistochemical staining of fixed brain sections.
Immunocytochemistry of cultured human Medium Spiny Neurons (MSNs)
By onThis protocol describes the immunolabelling of one or several protein targets on PFA-fixed cell culture on glass coverslips.
Accelerated Ovarian Failure AOF mouse model: a model of transitional menopause
By onThe novel model of AOF successfully replicates the human peri- and post-menopause stages, including erratic estrous cycles and hormone fluctuations, followed by undetectable estrogen levels.
Maintenance of the recording chamber for non-human primate (Chamber cleaning and dura scraping)
By onThis protocol explains two procedures to maintain the recording chamber in nonhuman primates with craniotomies:(1) chamber cleaning and (2) dura scraping.
Datasets associated with “Peripheral Neuronal Activation Shapes the Microbiome and Alters Gut Physiology”
By onDataset associated with the publication "Peripheral Neuronal Activation Shapes the Microbiome and Alters Gut Physiology"
Handling and behavioral training protocol for in vivo electrophysiological and optogenetic manipulation of basal ganglia neurons in awake head-fixed mice
By onThis protocol details the habituation of animals and behavioral training of animals for in vivo electrophysiology or optogenetic experiments.
Stereotactic Viral Injection into Subthalamic Nucleus in Mice
By onThis protocol describes the surgical procedure of injecting AAV viruses into the subthalamic nucleus of a mouse. It contains information regarding the stereotactic setup, surgery preparation, anesthesia, craniotomy, injection, and recovery.
Delivery of RNA Therapeutics: The Great Endosomal Escape!
By onRNA therapeutics show promise in treating various diseases by targeting specific genes without needing prior protein knowledge. Overcoming cellular defenses like endosomal entrapment is crucial for their widespread use in treating diseases.
Stereotaxic rat brain surgery for Substantia Nigra targeting
By onProtocol for stereotaxic rat brain surgery for Substantia Nigra targeting.
Fluorescent Immunolabelling for endogenous mouse LRRK2
By onThis protocol is used to label endogenous LRRK2 in mouse. It has been edited from West et al., 2014. Protocol optimized for fresh fixed brain tissue sectioned at 40 um.
Endosome isolation
By onSubcellular fractionation to isolate early and late endosomes (EEs and LEs) by performing a series of centrifugation steps.
Confocal imaging and digital image analysis
By onProtocol for confocal imaging on Nikon A1R microscope & digital image analysis is detailed.
Differentiation of human cortical neurons (CNs) from induced pluripotent stem cells (iPSCs) and their coculture with rat astrocytes
By onThis protocol described the production of human cortical neurons from induced pluripotent stem cells in cocultured with commercially available rat astrocytes.
Multiscale model of primary motor cortex circuits predicts in vivo cell type-specific, behavioral state-dependent dynamics
By onThe authors developed a multiscale model of mouse primary motor cortex with over 10,000 neurons and 30 million synapses. The model accurately predicted cell type-specific responses associated with behavioral states and experimental manipulations
Rapid iPSC inclusionopathy models shed light on formation, consequence, and molecular subtype of α-synuclein inclusions
By onThe authors developed an iPSC toolbox utilizing piggyBac-based or targeted transgenes to rapidly induce CNS cells with concomitant expression of aggregation-prone proteins.
Immunostaining Mouse Brain Tissue or Neuronal Cultures
By onThis protocol describes immunohistochemical and immunoctyochemical methods to analyze protein expression in mouse brain. In the protocol, we describe the fixation of tissue or cultured neurons, the immunostaining procedure, and collecting images for analysis.
Characterization of Splay Characteristics of Carbon Fiber Electrode Threads (CFETs) in Agar Brain Phantom
By onMethods to measure deflection characteristics of carbon fiber electrodes in agar brain phantoms.
