Aspiration of Fluid in Semi-Sealed Chamber
By onProtocol for post-op maintenance of electrode chambers emphasizes fluid aspiration and contamination prevention to reduce infection risk. Regular cleaning is crucial for asepsis and preventing complications like meningitis.
Aseptic, semi-sealed cranial chamber implants for chronic multi-channel neurochemical and electrophysiological neural recording in nonhuman primates
By onWe developed an implantable neural interface in monkeys that allows dual monitoring of electrical and chemical neural activity for long-term studies.
pAAV-pCAG-iCRE-2A-H2BGFP-WPRE (e.g. AAViCre-H2B-GFP)
By onCAG-driven Cre recombinase coupled with H2B-GFP for nuclear fluorescent labelling
pAAV-pCAG-Floxed-SynGFP,rev.-WPRE (e.g. AAV-flex-SynTag)
By onCAG-driven, Cre-dependent synaptophysin-tagged GFP for anterograde tracing of neuronal projections.
pAAV-pCAG-FRT(F3F5)-ChRmine3.3-p2A-oScarlet,rev.-WPRE (e.g. AAV-FRT-ChRmine-p2a-oScarlet)
By onPlasmid is CAG-driven, Flp-dependent ChRmine for optogenetic excitation of neurons with an oScarlet tag.
pAAV-pCAG-Floxed-FlpO-2A-H2BV5c.o.,rev.-WPRE (e.g. AAV-flex-FlpO-H2B-V5)
By onPlasmid is CAG-driven, Cre-dependent FlpO recombinase for H2B-V5.
Three antibody staining protocol for immunolabeling of mouse brain sections
By onThe protocol addresses factors affecting imaging of tissue sections: autofluorescence and consistency in antibody preparation for ideal results.
Synaptic analysis workflow using SEQUIN
By onProtocol for analyzing synaptic loci in neuroscience experiments using Zeiss Airyscan microscope. Includes identification, quantification, and localization of synaptic loci and nearby labels on neurites of the super-resolution images acquired
Stereotactic injection of viral vectors
By onHere we describe stereotactic injection of AAV into ventral tegmental area (VTA) of mice.
Combined RNAscope and immunohistochemistry labeling of fresh mouse midbrain tissue
By onHere we describe labeling coronal sections of mouse substantia nigra (SNc) and ventral tegmental area (VTA) for the gene Slc17a6 (VGLUT2) and virally-expressed histone-tagged GFP.
Neuromelanin and selective neuronal vulnerability to Parkinson’s disease
By onNeuromelanine (NM), a pigment in human catecholamine neurons, plays a crucial role in PD. We review the development of NM in dopamine versus noradrenaline neurons and focus on previously overlooked cellular organelles in NM format and function.
Dynamic basal ganglia output signals license and suppress forelimb movements-Datasets
By onThis repository contains the datasets and analysis files associated with the study by Falasconi, Kanodia, and Arber (2025).
Dynamic basal ganglia output signals license and suppress forelimb movements
By onBasal ganglia is fundamental to motor control and dysfunction is linked to motor deficits. Our results demonstrate the existence and function of highly specific and temporally precise movement representations in basal ganglia output circuitry.
Mouse Stereotaxic Surgery-Arber Lab
By onThis protocol describes the steps for performing stereotaxic surgery in mice. It is applicable to intracranial injections (e.g. virus, drug) and placement of implants (e.g. optical fibers, electrode arrays) into targeted regions of mouse brains.
Immunohistochemistry & Microscopy-Falasconi & Kanodia et al 2025
By onImmunohistochemistry and microscopy methods used in Falasconi & Kanodia et al 2025.
AAV Production Protocol-Arber Lab
By onAAV Production methods used in Falasconi & Kanodia et al 2025 and is the general protocol for Arber Lab.
Tissue Isolation of the Region Around Locus Coeruleus for Single Nucleus RNA Profiling
By onIsolating tissue from the peri-locus coeruleus region in mice for single nucleus RNA
Stereotaxic virus injection in the SNpc of rats
By onProtocol for stereotaxic virus injection in the substantia nigra pars compacta of rats
Optimized Stereotactic Injection Protocol for Targeting the Locus Coeruleus with Minimal Neurotoxicity
By onRefined stereotactic injection protocol targeting the Locus Coeruleus minimizes neurotoxicity.
Preparation of tissue punches of SNpc for the analyses of mitochondrial function
By onProtocol for the preparation of tissue punches of SNpc for the analyses of mitochondrial function
