Motor Behavioral Assessment
By onProtocol outlines motor behavior tests for Parkinson's disease in mice using open field, rotarod, cylinder, and balance beam tests. Mice were habituated for 30-40 min before tests conducted 3-4 weeks post-injections.
Mouse Whole Gut Transit Time
By onProtocol used in the Gradinaru lab for obtaining whole gut transit time for mice
Current safety recommendations for handling mouse and human αsynuclein pre-formed fibrils
By onThe paper outlines best practices in conducting research with α-synuclein fibrils. We highlight steps in which extra precautions should be taken and how to minimize exposure and potential risk associated with use of PFFs in scientific research.
Coronal cryosectioning of mouse brains
By onProtocol used in the Gradinaru lab for coronal cryosectioning of mouse brain tissue with a cryostat
Immunostaining Mouse Tissue
By onProtocol used in the Gradinaru lab for immunohistochemistry to analyze protein expression in the mouse brain
RNA extraction from organoids
By onProtocol used in the Gradinaru lab for RNA extraction from organoids
Copy of RNA extraction from mouse tissue
By onProtocol used in the Gradinaru lab for RNA extraction from mouse tissue
Genetically encoded self-assembling synuclein for tunable Parkinsonian pathology in vitro and in vivo
By onThis paper describes the self-assembling synuclein (SAS) system, a genetically-encoded tool for inducing temporally-controllable, tunable PD pathology in a wide range of in vitro and in vitro models.
Transfection of HEK cells with Lipofectamine 3000
By onProtocol used in the Gradinaru lab to transfect HEK cells with lipofectamine 3000
Western Blot with iBind System
By onProtocol used in the Gradinaru lab for western blot using the iBind Automated Western System
Primary Neuron Extraction
By onProtocol used in the Gradinaru lab to extract primary neurons from mouse on day E17
Mouse Open Field
By onProtocol used in the Gradinaru lab for general locomotion in rodents using open field arenas and Ethovision video tracking
sPAtial Neuronal Gene Expression Atlas (PANGEA) Application
By onPANGEA is a web app for visualizing brain regional gene expression data. It's a spatial atlas showing gene patterns related to neurons vulnerable to α-synuclein pathology.
Immunocytochemistry (Henderson Lab)
By onProtocol for immunocytochemistry on mammalian cells in 24 and 96 well plates.
Network analysis of α-synuclein pathology progression reveals p21-activated kinases as regulators of vulnerability
By onMisfolded α-synuclein accumulation in PD leads to pathogenic processes. We mapped α-synuclein pathology in mice to identify vulnerable regions and potential therapeutic targets, highlighting group II PAK inhibitors as promising for treating PD.
