Activation of transposable elements is linked to a region- and cell-type-specific interferon response in Parkinson’s disease
By onStudy explores transposable elements role in Parkinson's disease neuroinflammation. TE activation in specific brain regions correlates with innate immune responses, indicating potential involvement in chronic neuroinflammation and PD progression.
T cell purification and activation
By onThis protocol details the purification and activation of Mouse Naïve CD8+ T Cell from mouse spleen
Release Notes – ASAP CRN Cloud – Version 5.0.0
By onASAP CRN Cloud released version 5.0.0 which includes human metagenomics, gastrointestinal spatial transcriptomics, and new PMDBS data (ATAC seq and ParseBio). This release also introduced mouse proteomics, metabolomics, lipidomics, and metagenomics.
Protocol for efficient CRISPRi-mediated silencing of retrotransposons in human pluripotent stem cells
By onThis methods paper outlines silencing transposable elements in hiPSCs using CRISPRi. Describes gRNA design, validation via multiome approach. Enables functional studies on TE transcription in hiPSC models.
Adoptive Activated T cell Transfer Protocol
By onIsolation, in vitro activation, and intraperitoneal delivery of TCR-transgenic CD8+ T cells
Intracellular Cytokine Staining (ICS) Protocol for FACS Analysis
By onIntracellular staining of lymphocytic cells for Fluorescence-Activated Cell Sorting (FACS) analysis
A single Citrobacter rodentium infection in Pink1 knockout and wild type mice leads to regional blood-brain-barrier perturbation and limited microglial activation without dopamine neuron axon terminal loss
By onResearch suggests immune system activation may contribute to Parkinson’s disease development. Mild infections can increase BBB permeability, triggering brain inflammation in genetically susceptible individuals, potentially leading to PD pathology.
Dataset for “A single Citrobacter rodentium infection in Pink1 knockout and wild-type mice leads to regional blood-brain-barrier perturbation and limited microglial activation without dopamine neuron axon terminal loss”
By onImmune system activation may contribute to PD development. Infection increases BBB permeability in mice, leading to brain inflammation. This suggests infections could trigger PD in genetically susceptible individuals.
Generation of DATcrePink1 Knockout Mice Expressing tdTomato in Dopaminergic Neurons
By onProtocol details generation of DATcrePink1 mice expressing tdTomato in dopaminergic neurons. Covers Pink1 KO, HET, WT variants, animal housing, breeding, and genotyping procedures.
Semi-automated quantification of microglial CD68 and Iba1 immunohistochemistry signal in mouse brain slices
By onA standardized method for semi-automated detection and quantification of CD68 and Iba1 in brain sections using image acquisition, AI segmentation, and data analysis.
Automated Quantification of Dopaminergic Neurons in Floating Sections V.1
By onAutomated method for detecting and measuring dopaminergic neurons in brain sections using image capture, AI segmentation, and data analysis in SNc and VTA regions.
STING dampens the unfolded protein response to enable the presentation of self antigens on MHC-I during inflammation
By onSTING pathway regulates antigen presentation through the UPR, impacting immune response in PD and autoimmune diseases. Targeting STING and UPR could be therapeutic.
Dataset for “STING dampens the unfolded protein response to enable the presentation of self-antigens on MHC-I during inflammation”
By onSTING pathway activation impacts antigen presentation through the UPR and may be a target for therapeutic intervention in PD and autoimmune diseases.
Protocols for “LRRK2 mutations block NCOA4 trafficking upon iron overload leading to ferroptotic death”
By onLRRK2-G2019S affects iron homeostasis in macrophages by dysregulating iron-related proteins. This leads to impaired ferritinophagy, increased oxidative stress, and cell death, highlighting LRRK2's role in iron regulation in Parkinson's Disease.
An LRRK2 variant blocks NCOA4 trafficking upon iron overload, leading to ferroptotic death
By onWe examined the impact of mutant LRRK2-G2019S on iron homeostasis within a model macrophage cell line. Results revealed a dysregulation of iron-related proteins in steady state and a complete block in the degradation of the ferritinophagy NCOA4.
Protocols for “STING dampens the unfolded protein response to enable the presentation of self-antigens on MHC-I during inflammation”
By onSTING pathway regulates MitAP through the UPR, establishing it as a key immune regulator in autoimmunity and PD.
