L1-silenced iPSC line (HS1_L1HS-CRISPRi_g1)
By onHuman iPSCs were modified with CRISPRi to target LINE-1 retrotransposons, creating stable cell lines with different genetic backgrounds and guide RNAs. These resources are detailed in Adami et al. (DOI: 10.1016/j.xgen.2025.100979).
L1-silenced iPSC line (HS1_L1HS-CRISPRi_g2 )
By onHuman iPSCs were modified with CRISPRi to target LINE-1 retrotransposons, creating stable cell lines with different genetic backgrounds and guide RNAs. These resources are detailed in Adami et al. (DOI: 10.1016/j.xgen.2025.100979).
L1-silenced iPSC line – Control (HS1_L1HS-CRISPRi_LacZ )
By onHuman iPSCs were modified with CRISPRi to target LINE-1 retrotransposons, creating stable cell lines with different genetic backgrounds and guide RNAs. These resources are detailed in Adami et al. (DOI: 10.1016/j.xgen.2025.100979).
L1-silenced iPSC line (HS2_L1HS-CRISPRi_g1)
By onHuman iPSCs were modified with CRISPRi to target LINE-1 retrotransposons, creating stable cell lines with different genetic backgrounds and guide RNAs. These resources are detailed in Adami et al. (DOI: 10.1016/j.xgen.2025.100979).
L1-silenced iPSC line (HS2_L1HS-CRISPRi_LacZ)
By onHuman iPSCs were modified with CRISPRi to target LINE-1 retrotransposons, creating stable cell lines with different genetic backgrounds and guide RNAs. These resources are detailed in Adami et al. (DOI: 10.1016/j.xgen.2025.100979).
Microglia differentiation from human pluripotent stem cells
By onMicroglia are crucial for brain development and maintaining homeostasis. They are involved in neurodegenerative diseases and can be generated from human stem cells using established protocols.
Stimulation of DA neurons and microglia
By onThis protocol details how to stimulate microglia with IFN-gamma, poly-IC and TNF-alpha.
iPSC QC (Adami et al, 2025)
By onSummary table and data from iPSC quality control experiments in Adami et al. 2025 are accessible for review.
Reply to: Is Gauchian genotyping of GBA1 variants reliable?
By onSummarizing a study by N. Tayebi et al. in Communications Biology, published in 2025.
Characterizing Parkinson’s Disease Clinical and Biomarker Interactions in REM Sleep Behavior Disorder
By ona-syn SAA positivity, DaT positivity, and hyposmia are highly associated with each other. MDS Prodromal PD Probability scores may be useful predictors of near-term progression, and thus as a stratification factor in clinical research study design
Inflamed Microglia like Macrophages in the Central Nervous System of Prodromal Parkinson′s Disease
By onWe investigated the role of inflammation in the pathogenesis of prodromal PD performing single-cell RNAseq analysis of CSF and blood from 111 individuals.
Article: PINK1 deficiency rewires early immune responses in a mouse model of Parkinson’s disease triggered by intestinal infection
By onPINK1 KO mice show PD-like symptoms post intestinal infections, with myeloid cells dysregulation and T cell response early after infection. PINK1 plays a key role in gut immune functions linked to early PD mechanisms.
Supporting data for “PINK1 deficiency rewires early immune responses in a mouse model of Parkinson’s disease triggered by intestinal infection”
By onData associated with “PINK1 deficiency rewires early immune responses in a mouse model of Parkinson’s disease triggered by intestinal infection”
Immunostaining and Western blot images
By onRaw immunostaining and western blot images included in Adami et al, 2025.
Baseline α–synuclein Seeding Activity and Disease Progression Code
By onThe project analyzes baseline α-synuclein seeding activity and Parkinson's disease progression.
Soluble Immune Factor Profiles in Blood and CSF Associated with LRRK2 Mutations and Parkinson’s Disease
By onThis preprint explores immune factors in Parkinson's disease linked to LRRK2 mutations. Serum showed elevated SDF-1 alpha and TNF-RII levels in LRRK2 carriers, while CSF had reduced immune markers. PD patients displayed reduced CSF inflammation.
Severe GBA1 variants drive the GBA-PD clinical phenotype: implications for counselling and clinical trials
By onVariants in the GBA1 gene are common genetic risk factors for Parkinson's disease. A study compared clinical phenotypes in GBA-PD and idiopathic PD patients, finding that only severe GBA-PD cases show a more severe clinical profile.
Exploring the relationship between GBA1 host genotype and gut microbiome in the GBA1L444P/WT mouse model: Implications for Parkinson disease pathogenesis
By onHeterozygous *GBA1* variants are common in Parkinson's disease. This study found no significant impact of the *GBA1* L444P variant on gut microbiome composition in mice, suggesting other factors may contribute to disease penetrance.
Immunohistochemistry (Brain organoids)
By onProtocol for preparing brain organoid slides and performing immunostainings to analyze protein expression and localization in the 3D model.
