ASAP CRN Cloud Release Notes – Version 4.0.0
By onASAP CRN Cloud released version 4.0.0 with expanded datasets including Human Postmortem-derived Brain Sequencing and Mouse datasets. New harmonized collections and individual datasets were added, enhancing research possibilities.
Myeloid PINK1 represses mtDNA release and immune signaling that impacts neuronal pathology in patient-derived idiopathic PD models
By onPINK1 represses mtDNA release and STING/NF-κB activation in peripheral macrophages of PD model laying the foundation for understanding PINK1-related peripheral mechanisms in idiopathic PD and providing targets for further therapy development
Protocols Suite — SH-SY5Y / iCell® Neurons / High-Content Imaging
By onMaintenance of SH-SY5Y cells with SNCA overexpression in DMEM/F-12, differentiation with RA/BDNF, and culture of iCell DopaNeurons with SNCA mutation. Treatment and immunostaining for downstream assays using specific protocols and analysis tools.
GCase activity assay
By onProtocol measures GCase activity using 4-MUG substrate. GCase protein from a cellular model is diluted in acidic buffer to mimic lysosome pH. Adding lipid/detergent maintains enzyme activity. Taurocholate bile salt is included in the protocol.
Immunofluorescencent Staining of A-synuclein, TFEB, GCase and LAMP1 in treated cultured cells
By onProtocol used to detect a-synuclein, TFEB, GCase, and LAMP1 via immunofluorescence in SH-SY5Y cells with mutant SNCA gene.
Protocol — DQ™ Red BSA Lysosomal Activity Assay HCS Analysis
By onLysosomal proteolysis can be measured using self-quenched DQ™ Red BSA, followed by confocal imaging for spot-based quantification.
Protocol — Quantitative Real-Time PCR (qRT-PCR): 3K-SNCA cells
By onGene expression in 3K-SNCA SH-SY5Y cells was analyzed using Qiagen RNA extraction and TaqMan qRT-PCR on QuantStudio 1 platform for differentiated and undifferentiated cells.
Protocol — LysoTracker® Red DND-99 Live-Cell Staining 26 HCS Analysis
By onText summary: Live-cell lysosomal staining done with LysoTracker Red DND-99, followed by high-content confocal imaging using Opera Phenix Plus, and automated quantification in Harmony/Columbus.
Processing Serum, Plasma, Plasma EDTA from Whole Blood
By onProtocols cover serum, plasma, and plasma EDTA processing from whole blood samples.
Isolation of Classical Monocytes from PBMCs (MB Kit No. 130-117-337)
By onProtocol isolates classical monocytes from peripheral blood mononuclear cells.
PBMCs isolation with CT Tubes (8mL Whole blood)
By onProtocol isolates peripheral blood mononuclear cells using CPT tubes.
Metagenomics sequencing data of publication doi.org/10.3389/fnins.2025.1546203
By onThe study explores the connection between *GBA1* gene variants and gut microbiome in a mouse model, shedding light on Parkinson's disease development.
MSP abundance table associated with doi.org/10.3389/fnins.2025.1546203
By onSummary: The table displays the abundance of microbial species in the gut of GBA1 L444P/WT mouse model, exploring the connection between GBA1 host genotype and gut microbiome in Parkinson's disease research.
Read Alignment table associated with doi.org/10.3389/fnins.2025.1546203
By onSummary: The publication explores the link between the *GBA1* host genotype and gut microbiome in the *GBA1*L444P/WT mouse model, shedding light on Parkinson's disease development.
Clinical data associated with doi.org/10.1038/s41531-025-01063-3
By onStudy shows severe *GBA1* variants contribute to Parkinson's Disease phenotype, impacting counseling and clinical trials. Findings available at doi.org/10.1038/s41531-025-01063-3.
Microbiome signature of Parkinson disease in healthy and genetically at-risk individuals
By onParkinson's disease (PD) is disabling and costly. *GBA1* variants increase PD risk. Gut microbiome changes can predict PD progression and identify those at risk, suggesting potential for disease modification through nutrition.
Code R script related to doi.org/10.1038/s41531-025-01063-3
By onSevere GBA1 variants are linked to Parkinson's disease, impacting counseling and clinical trials. Access R script at doi.org/10.1038/s41531-025-01063-3 for more details.
ASAP CRN Cloud Release Notes – Version 2.0.0
By onVersion: 2.0.0 Release Date: December 11, 2024 This release notes document provides a concise overview of the updates and enhancements introduced in Version 2.0.0 of the CRN Cloud platform.
