Microsomal membrane isolation from cell culture
By savannah onThis protocol describes microsomal membrane isolation from cell culture.
RBG motif bridge-like lipid transport proteins: Structure, functions, and open questions
By savannah onMutations in some of these proteins result in neurodegenerative diseases. The authors review the known properties and well-established or putative physiological roles of these proteins, and highlight the many questions that remain open about their functions.
Local diffusion in the extracellular space of the brain
By savannah onThe authors highlight emerging technological advances to respectively interrogate and model diffusion through the ECS, and point out how these may contribute in resolving the remaining enigmas of the ECS.
Neuromelanin accumulation drives endogenous synucleinopathy in non-human primates
By savannah onPublished: Evidence is provided showing that intracellular aggregation of endogenous alpha-synuclein is triggered by NMel accumulation; therefore any therapeutic approach intended to decrease NMel levels may provide appealing choices for the successful implementation of novel PD therapeutics. View original preprint.
Non-ablative disease-modifying effects of magnetic resonance-guided focused ultrasound in neuromelanin-producing parkinsonian rodents
By savannah onPreprint: The authors' findings indicate that tFUS treatment applied at prodromal/early disease stages provides by itself extended structural and functional preservation of the nigrostriatal pathway in neuromelanin-producing parkinsonian rats without causing overt neuronal damage.
Brain slice physiology and optogenetics
By savannah onThis protocol describes Chu Lab's standard external solutions for electrophysiology.
Brain slice preparation for electrophysiology recording
By savannah onThis protocol describes brain slice preparation for electrophysiology recording.
Immunohistochemistry Protocol – Chu Lab (Wichmann)
By savannah onThis protocol describes immunohistochemistry.
Confocal imaging and digital image analysis
By savannah onThis protocol describes confocal imaging on a Nikon A1R confocal microscope and digital image analysis.
Multiscale model of primary motor cortex circuits predicts in vivo cell type-specific, behavioral state-dependent dynamics
By savannah onThis dataset includes experimental data used to constrain and validate the model and model simulation output data in the Cell Reports publication: Dura-Bernal et al., 2023, "Multiscale model of primary motor cortex circuits predicts in vivo cell type-specific, behavioral state-dependent dynamics."
Quantification of TH immunoreactivity
By savannah onThis protocol describes the quantification of TH immunoreactivity.
Reduced thalamic excitation to motor cortical pyramidal tract neurons in parkinsonism
By savannah onPreprint: The authors’ data suggest that cell subtype- and synapse-specific adaptations in M1 contribute to altered cortical outputs in parkinsonism and are important aspects of PD pathophysiology.
Pseudogenes limit the identification of novel common transcripts generated by their parent genes
By savannah onGenomic sequences with high sequence similarity, such as parent-pseudogene pairs, cause short sequencing reads to align to multiple locations, thus complicating genomic analyses. However, their impact on transcriptomic analyses, including the estimation of gene expression and transcript annotation, has been less studied. The authors investigated the impact of pseudogenes on transcriptomic analyses.
SoRA microscopy protocol for imaging oligomers in human brain tissue
By savannah onThis protocol gives a step-by-step guide to imaging oligomers in human brain tissue using a spinning disk confocal microscope. The microscope used for this protocol was a commercial set up produced by 3i; however the techniques described can be used as general guidelines with alternative microscopes.
Quantifying the LAMP1 positive puncta
By savannah onThis protocol describes quantifying the LAMP1 positive puncta.
Nuclear cytoplasmic fractionation
By savannah onThis protocol describes nuclear cytoplasmic fractionation.
