twichma/Sequence-and-pattern-detection: Sequence and pattern detection code
By onThe code provided is for analyzing sequences and patterns.
Basal ganglia neurons in healthy and parkinsonian primates generate recurring sequences of spikes
By onBasal ganglia neurons fire in recognizable, repeating sequences of interspike intervals (ISIs), the incidence of which is influenced by the induction of Parkinsonism.
Rodent models based on endolysosomal genes involved in PArkinson’s disease
By onGenes linked to endolysosomal function are connected to Parkinson's disease, suggesting a role in its development. Rodent models targeting these genes like LRRK2 and GBA1 show parkinsonian features, aiding in understanding and treating the disease.
Soluble and insoluble A-SYN fractionation
By onSoluble/insoluble alpha-synuclein fractionation is a technique used to separate different forms of the alpha-synuclein protein based on their solubility properties.
Macros and pipelines for analysis of image analysis
By onImageJ macros and pipelines for analysis of pUb puncta, Keima Ratio, p62nuclei and Parkin recruitment to mitochondria.
Fixation of HeLa-M cells expressing Halo and SNAP fusion proteins conjugated to ligands
By onProtocol describing fixation of HeLa-M cells expressing Halo and SNAP fusion proteins conjugated to ligands.
Purification and analysis of SKP1-FBXO7 complexes
By onProtocol for the biochemical purification and analysis of SKP1-FBXO7 complexes.
Preparation of LRRK2 RCKW cryo-EM grids
By onThis is Leschziner's Lab updated protocol for making cryo-EM grids for LRRK2 RCKW. This protocol, when using lower protein concentration, results in better monomer and dimer formation than the old protocol.
Confocal imaging on Nikon AXR confocal microscope
By onA step by step protocol to acquire images on Nikon AXR confocal set-up.
powereQTL – An R shiny application for calculating sample size and power of bulk tissue and single-cell eQTL analysis
By onEasy-to-use web application for calculating sample size and power of bulk tissue and single-cell eQTL analysis
Endogenous tagging of the YIPF4 gene with mNEON Green and imaging of these cells by microscopy
By onProtocol for tagging YIPF4 gene with mNEON in cells using CRISPR. Method involves editing N-terminus of YIPF4 in WT and FIP200-/- HEK293T cells. Imaging mNEON-YIPF4 in Golgi with GOLGB1 marker is also detailed.
iNeuron differentiation from human iPSCs
By onThe authors adapted a previously-described method for differentiating iPSCs stably expressing mNGN2 at a safe-harbor locus into human excitatory glutamatergic neurons.
Determination of free and protein-bound DA and NE and their metabolites and oxidation products by UPLC-MS/MS method
By onProtocol for the determination of free and protein-bound DA and NE and their metabolites and oxidation products by UPLC-MS/MS method.
pBPK1520-HEK3-CTTins-ng
By onIt can be used to introduce HEK3 CTT insertion using prime editing, PE3 approach. It serves as a good positive control for prime editing.
