Lentiviral vector plasmid for overexpression of ATP10B (EmGFP)
By onLentiviral vector plasmid for overexpression of ATP10B (EmGFP) validated for use in cell culture
Lentiviral vector plasmid for overexpression of ATP10B (E210A)
By onLentiviral vector plasmid for overexpression of ATP10B (E210A) validated for use in cell culture
LRRK2 kinase inhibition reverses G2019S mutation-dependent effects on tau pathology spread
By onMutations in LRRK2 are the most common cause of familial Parkinson’s disease. This work supports a protective role of LRRK2 kinase inhibition in G2019S carriers and provides a workflow for systematic evaluation of brain-wide phenotypes
Reduction of a-synuclein aggregates by PIKfyve inhibition via TFEB-mediated lysosomal biogenesis in a Parkinson disease model
By onIn the present study, we exploited the SH-SY5Y cell model overexpressing a pro-aggregation form of alpha-synuclein to investigate the efficacy of PIKfyve-mediated lysosomal biogenesis, through TFEB, as potential target for Parkinson therapy.
Saliva metagenomic data related to “Evaluation of an Adapted Semi-Automated DNA Extraction for Human Salivary Shotgun Metagenomics”
By onIn this study, we proposed a semi-automated DNA extraction protocol for human salivary samples collected with a commercial kit, and compared the outcomes with the DNA extraction recommended by the manufacturer. This study demonstrates that our semi-automated protocol is suitable for shotgun metagenomic analysis, by significantly producing higher DNA fragment sizes, while allowing for improved sample treatment logistics with reduced technical variability and without compromising the structure of the oral microbiome.
Immunoblots
By onThe steps of this protocol are similar for different sources of protein such as cell lysate, cytosol, or membrane samples, and EV samples.
shRNA knockdown
By onThis protocol describes a standard procedure to generate a stable cell line for a DNAJC5 knockdown using shRNA.
Differentiation of SH-SY5Y cells
By onThis protocol describes a standard procedure to differentiate SH-SY5Y cells into dopaminergic neurons using retinoic acid
Mitochondria purification
By onThis protocol describes how to purify mitochondria from cell culture.
Strep-pull down assay
By onStrep pull down assay for FIP200(NTD)-TSF WT or mutants were co-transfected with GST-ATG13(363-517) and/or ULK1(MIT)-MBP.
Protein Digestion and Mass Spectrometry Analysis Protocol
By onThis protocol details the procedure for the trypsin digestion of PI3KC3-C1 and subsequent analysis of digested peptides using LC-MS/MS.
Plasmid Construction Protocol
By onThis protocol details plasmid construction in general terms for the insertion of genes into the pCAG mammalian expression vector.
Preparing mitochondrial samples for immunoblot analysis
By onProtocol for preparation of mitochondrial samples for immunoblot analysis.
Molecular Dynamics Simulations
By onThis protocol details molecular dynamics simulations of PI3KC3-C1 on a lipid membrane.
HPLC Analysis of Nucleotides
By onIon-pair reversed phase HPLC analysis of the nucleotide bound to PI3KC3-C1.
Electrospray ionization analysis of eluted nucleotides
By onThis protocol describes the denaturation of PI3KC3-C1, and subsequent analysis of eluted nucleotides by electrospray ionization mass spec.
Cryo-EM sample preparation for PI3KC3-C1~RAB1A complex
By onThis protocol details cryo-EM sample preparation for PI3KC3-C1~RAB1A complex.
Synthesis of 1-(3-Chlorophenethyl)-3-cyclopentylpyrimidine-2,4,6-(1H,3H,5H)-trione
By on1-(3-chlorophenethyl)-3-cyclopentylpyrimidine-2,4,6-(1H,3H,5H)-trione (8) is a potent and highly selective Ca(V)1.3 L-type calcium channel antagonist. This protocol is an adaption of Kang et al. 2012 's method of the synthesis of this chemical.
LBD-case-case-GWAS
By onCode used in article: Investigation of the genetic aetiology of Lewy body diseases with and without dementia.
