Measuring mitophagy via FACS with mtKeima reporter
By onPreparation of samples for measuring mitophagy levels using mtKeima reporter by fluorescence activated cell sorting (FACS).
Analyzing oxygen consumption of isolated mitochondria using the Seahorse XFe96 analyzer
By onProtocol for analyzing oxygen consumption of isolated mitochondria using the Seahorse XFe96 analyzer.
Analyzing cellular ATP levels
By onProtocol for analysis of cellular ATP levels using the Promega Mitochondrial ToxGlo Kit.
qRT-PCR sample preparation
By onProtocol for qRT-PCR sample preparation for analysis using a RotorGeneQ machine (Qiagen).
Immunofluorescence Assay (IFA)
By onThis protocol details the procedure of immunofluorescence assay (IFA).
Temporal landscape of mitochondrial proteostasis governed by the UPRmt
By onSource microscopy data associated with 10.1126/sciadv.adh8228
Temporal landscape of mitochondrial proteostasis governed by the UPRmt_NGS data_Part 1
By onNGS data associated with Uoselis et al., 2023.
Temporal landscape of mitochondrial proteostasis governed by the UPRmt_NGS data_Part 2
By onPart 2 of NGS data associated with Uoselis et al., 2023.
Generation of A549 PPM1H BromoTag CRISPR/CAS9 knock-in cell line
By onThis protocol details the generation of A549 PPM1H BromoTag CRISPR/CAS9 knock-in cell line.
Control of mitophagy initiation and progression by the TBK1 adaptors NAP1 and SINTBAD
By onMitophagy maintains mitochondrial health by degrading damaged mitochondria. TBK1 adaptors NAP1 and SINTBAD regulate OPTN-driven mitophagy initiation and NDP52-driven progression, balancing pathway activity for efficient functioning.
Cell Culture
By onThis protocol describes routine cell culture maintenance as described in Adriaenssens & Nguyen et al., 2023 (BioRxiv)
Plasmid Construction and Gibson Cloning
By onThis protocol describes plasmid construction as described in Adriaenssens & Nguyen et al., 2023 (BioRxiv)
Multiple genome alignment in the telomere-to-telomere assembly era
By onThis review provides an overview of the algorithmic template that most multiple genome alignment methods follow.
Next-generation sequencing of AAV.CAP-Mac enrichment from Chuapoco et al. (2023)
By onDataset of next-generation sequencing of enrichment of AAV.CAP-Mac in various tissues from the publication: Chuapoco, M.R., Flytzanis, N.C., Goeden, N. et al. Adeno-associated viral vectors for functional intravenous gene transfer throughout the non-human primate brain. Nat. Nanotechnol. (2023). https://doi.org/10.1038/s41565-023-01419-x
Structural pathway for PI3-kinase regulation by VPS15 in autophagy
By onCryo-EM analysis reveals activation pathway of PI3KC3-C1 in atomistic detail.
Biochemical consequences of glucocerebrosidase 1 mutations in Parkinson’s disease
By onPerspective on the biochemical consequences of glucocerebrosidase 1 mutations in Parkinson’s disease.
Sex Distribution of GBA1 Variants Carriers with Dementia with Lewy Bodies and Parkinson’s Disease
By onSex Distribution of GBA1 Variants Carriers with Dementia with Lewy Bodies and Parkinson's Disease
R scripts for analysis of publication “Sex distribution of GBA1 Variants Carriers with Dementia with Lewy Bodies and Parkinson Disease”
By onR scripts for analysis of publication "Sex distribution of GBA1 variant carriers with dementia with Lewy Bodies and Parkinson's disease."
Salivary collection participant instructions using OMNIgene ORAL OM 501 device
By onSalivary collection participant instructions using OMNIgene ORAL OM 501 device
A topographical atlas of α-synuclein dosage and cell type-specific expression in adult mouse brain and peripheral organs
By onThis atlas provides much-needed insight into the cellular topography of αSyn, and provides a quantitative map to test assumptions about the role of αSyn in network vulnerability in PD and other αSynucleinopathies.
