Testing AAVrg-hSyn-mCherry in the Spinal Cord of Rhesus Macaques
By onViral Vector: Two rhesus macaque animals were tested for AAVrg-hSyn-mCherry in the spinal cord. The authors observed abundant mCherry-positive neurons in expected targets (i.e., motor cortical regions known to project to the spinal cord).
Generation of stable cell lines using viral infection
By onProtocol for generation and precipitation of retrovirus, and infection of HeLa cells to generate stable cell lines.
Mitophagy induction using Oligomycin/Antimycin A
By onMitophagy induction in HeLa cells using Oligomycin/Antimycin A.
Induction of starvation stress
By onProtocol describing the induction of starvation stress using EBSS in HeLa cells
Mitophagy induction using Difereprone
By onProtocol describing the procedure for mitophagy induction in HeLa cells using Difereprone (DFP).
Preparing whole cell samples for immunoblot analysis
By onProtocol for preparation of HeLa cell lysates for immunoblot analysis.
Western blotting using the BioRad Criterion Blotter system
By onProtocol for performing an SDS-PAGE Western blot analysis using the BioRad Criterion Blotter system.
Preparation of soluble and insoluble mitochondrial protein fractions for immunoblotting
By onProtocol describing the preparation procedure of soluble and insoluble mitochondrial protein fractions from HeLa cells for immunoblotting.
Mitochondrial isolation from HeLa cells
By onMitochondrial isolation and quantification from HeLa cells.
Preparation of soluble and insoluble mitochondrial protein fractions for mass spectrometry analysis
By onProtocol describing preparation of soluble and insoluble mitochondrial protein fractions from HeLa cells for mass spectrometry analysis.
Calculating mitochondrial protein solubility
By onProtocol for generating solubility calculations for mitochondrial proteins from starting 'soluble' and 'insoluble' fraction mass spectrometry data, using MaxQuant and Perseus software pipelines.
Reverse-phase high pH fractionation (using Thermo Fisher, Cat# 84868)
By onThis protocol uses the Pierce™ High pH Reversed-Phase Peptide Fractionation Kit (Thermo Fisher, Cat# 84868)
Measuring mitophagy via FACS with mtKeima reporter
By onPreparation of samples for measuring mitophagy levels using mtKeima reporter by fluorescence activated cell sorting (FACS).
Analyzing oxygen consumption of isolated mitochondria using the Seahorse XFe96 analyzer
By onProtocol for analyzing oxygen consumption of isolated mitochondria using the Seahorse XFe96 analyzer.
Analyzing cellular ATP levels
By onProtocol for analysis of cellular ATP levels using the Promega Mitochondrial ToxGlo Kit.
qRT-PCR sample preparation
By onProtocol for qRT-PCR sample preparation for analysis using a RotorGeneQ machine (Qiagen).
Immunofluorescence Assay (IFA)
By onThis protocol details the procedure of immunofluorescence assay (IFA).
Temporal landscape of mitochondrial proteostasis governed by the UPRmt
By onSource microscopy data associated with 10.1126/sciadv.adh8228
Temporal landscape of mitochondrial proteostasis governed by the UPRmt_NGS data_Part 1
By onNGS data associated with Uoselis et al., 2023.
