Preparation of soluble and insoluble mitochondrial protein fractions for mass spectrometry analysis
By onProtocol describing preparation of soluble and insoluble mitochondrial protein fractions from HeLa cells for mass spectrometry analysis.
Preparation of soluble and insoluble mitochondrial protein fractions for immunoblotting
By onProtocol describing the preparation procedure of soluble and insoluble mitochondrial protein fractions from HeLa cells for immunoblotting.
Mitochondrial isolation from HeLa cells
By onMitochondrial isolation and quantification from HeLa cells.
Western blotting using the BioRad Criterion Blotter system
By onProtocol for performing an SDS-PAGE Western blot analysis using the BioRad Criterion Blotter system.
Preparing whole cell samples for immunoblot analysis
By onProtocol for preparation of HeLa cell lysates for immunoblot analysis.
Induction of starvation stress
By onProtocol describing the induction of starvation stress using EBSS in HeLa cells
Immunofluorescence Assay (IFA)
By onThis protocol details the procedure of immunofluorescence assay (IFA).
qRT-PCR sample preparation
By onProtocol for qRT-PCR sample preparation for analysis using a RotorGeneQ machine (Qiagen).
Analyzing oxygen consumption of isolated mitochondria using the Seahorse XFe96 analyzer
By onProtocol for analyzing oxygen consumption of isolated mitochondria using the Seahorse XFe96 analyzer.
Analyzing cellular ATP levels
By onProtocol for analysis of cellular ATP levels using the Promega Mitochondrial ToxGlo Kit.
Measuring mitophagy via FACS with mtKeima reporter
By onPreparation of samples for measuring mitophagy levels using mtKeima reporter by fluorescence activated cell sorting (FACS).
Control of mitophagy initiation and progression by the TBK1 adaptors NAP1 and SINTBAD
By onMitophagy maintains mitochondrial health by degrading damaged mitochondria. TBK1 adaptors NAP1 and SINTBAD regulate OPTN-driven mitophagy initiation and NDP52-driven progression, balancing pathway activity for efficient functioning.
Generation of A549 PPM1H BromoTag CRISPR/CAS9 knock-in cell line
By onThis protocol details the generation of A549 PPM1H BromoTag CRISPR/CAS9 knock-in cell line.
Temporal landscape of mitochondrial proteostasis governed by the UPRmt_NGS data_Part 2
By onPart 2 of NGS data associated with Uoselis et al., 2023.
Temporal landscape of mitochondrial proteostasis governed by the UPRmt_NGS data_Part 1
By onNGS data associated with Uoselis et al., 2023.
Temporal landscape of mitochondrial proteostasis governed by the UPRmt
By onSource microscopy data associated with 10.1126/sciadv.adh8228
Structural pathway for PI3-kinase regulation by VPS15 in autophagy
By onCryo-EM analysis reveals activation pathway of PI3KC3-C1 in atomistic detail.
Next-generation sequencing of AAV.CAP-Mac enrichment from Chuapoco et al. (2023)
By onDataset of next-generation sequencing of enrichment of AAV.CAP-Mac in various tissues from the publication: Chuapoco, M.R., Flytzanis, N.C., Goeden, N. et al. Adeno-associated viral vectors for functional intravenous gene transfer throughout the non-human primate brain. Nat. Nanotechnol. (2023). https://doi.org/10.1038/s41565-023-01419-x
Multiple genome alignment in the telomere-to-telomere assembly era
By onThis review provides an overview of the algorithmic template that most multiple genome alignment methods follow.
