Lentiviral vector plasmid for overexpression of ATP10B (G671R/N865K)
By onLentiviral vector plasmid for overexpression of ATP10B (G671R/N865K) validated for use in cell culture
Lentiviral vector plasmid for overexpression of ATP10B (E993A)
By onLentiviral vector plasmid for overexpression of ATP10B (E993A) validated for use in cell culture
Lentiviral vector plasmid for overexpression of ATP10B (I1038T)
By onLentiviral vector plasmid for overexpression of ATP10B (I1038T) validated for use in cell culture
Lentiviral vector plasmid for knock down of ATP10B (miR5 Hs)
By onLentiviral vector plasmid for knock down of ATP10B (miR5, Hs) validated for use in cell culture
Single-cell somatic copy number variants in brain using different amplification methods and reference genomes
By onSomatic mutations in the brain are well-known, requiring single-cell whole genome amplification before sequencing. PicoPLEX, MDA, and PTA whole genome amplification methods were compared on brain nuclei, showing different properties.
Co-immunoprecipitation
By onThis protocol describes a common procedure to perform co-immunoprecipitation
Medium fractionation and EV preparation
By onThis protocol describes the characterization of the extracellular alpha-synuclein and DNAJC5
CRISPR/Cas9 genome editing
By onThis protocol describes the generation of DNAJC5 KO using CRISPR/Cas9 edition
In vitro depalmitoylation assay
By onThis protocol describes the in vitro depalmitoylation assay of DNAJC5.
Membrane and cytosol fractionation
By onThis protocol describes membrane and cytosol fractionation of cells expressing different DNAJC5 isoforms.
Lentiviral vector plasmid for overexpression of ATP10B (EmGFP)
By onLentiviral vector plasmid for overexpression of ATP10B (EmGFP) validated for use in cell culture
Lentiviral vector plasmid for overexpression of ATP10B (E210A)
By onLentiviral vector plasmid for overexpression of ATP10B (E210A) validated for use in cell culture
LRRK2 kinase inhibition reverses G2019S mutation-dependent effects on tau pathology spread
By onMutations in LRRK2 are the most common cause of familial Parkinson’s disease. This work supports a protective role of LRRK2 kinase inhibition in G2019S carriers and provides a workflow for systematic evaluation of brain-wide phenotypes
Reduction of a-synuclein aggregates by PIKfyve inhibition via TFEB-mediated lysosomal biogenesis in a Parkinson disease model
By onIn the present study, we exploited the SH-SY5Y cell model overexpressing a pro-aggregation form of alpha-synuclein to investigate the efficacy of PIKfyve-mediated lysosomal biogenesis, through TFEB, as potential target for Parkinson therapy.
Saliva metagenomic data related to “Evaluation of an Adapted Semi-Automated DNA Extraction for Human Salivary Shotgun Metagenomics”
By onIn this study, we proposed a semi-automated DNA extraction protocol for human salivary samples collected with a commercial kit, and compared the outcomes with the DNA extraction recommended by the manufacturer. This study demonstrates that our semi-automated protocol is suitable for shotgun metagenomic analysis, by significantly producing higher DNA fragment sizes, while allowing for improved sample treatment logistics with reduced technical variability and without compromising the structure of the oral microbiome.
Immunoblots
By onThe steps of this protocol are similar for different sources of protein such as cell lysate, cytosol, or membrane samples, and EV samples.
shRNA knockdown
By onThis protocol describes a standard procedure to generate a stable cell line for a DNAJC5 knockdown using shRNA.
Differentiation of SH-SY5Y cells
By onThis protocol describes a standard procedure to differentiate SH-SY5Y cells into dopaminergic neurons using retinoic acid
Mitochondria purification
By onThis protocol describes how to purify mitochondria from cell culture.
Strep-pull down assay
By onStrep pull down assay for FIP200(NTD)-TSF WT or mutants were co-transfected with GST-ATG13(363-517) and/or ULK1(MIT)-MBP.
