Collection of protocols of Team Deleidi used in the publication: “LRRK2 kinase activity regulates GCase level and enzymatic activity differently depending on cell type in Parkinson’s disease”
By savannah onCollection of protocols of Team Deleidi used in the publication: "LRRK2 kinase activity regulates GCase level and enzymatic activity differently depending on cell type in Parkinson’s disease."
Glucocerebrosidase, a Parkinson´s disease-associated protein, is imported into mitochondria and regulates complex I assembly and function
By savannah onRaw data files used for the manuscript "Glucocerebrosidase, a Parkinson´s disease-associated protein, is imported into mitochondria and regulates complex I assembly and function." https://www.researchsquare.com/article/rs-1521848/v1
Who is at risk of Parkinson’s disease? Refining the preclinical phase of GBA1 and LRRK2 variant carriers: a clinical, biochemical, and imaging approach
By savannah onGenetic variants in GBA1 and LRRK2 genes are the commonest genetic risk factor for Parkinson’s disease (PD); however, the preclinical profile of GBA1 and LRRK2 variant carriers who will develop PD is unclear. This review aims to highlight the more sensitive markers that can stratify PD risk in non-manifesting GBA1 and LRRK2 variant carriers.
LRRK2-G2019S synergizes with aging and low-grade inflammation to promote gut and peripheral immune cell activation that precede nigrostriatal degeneration
By savannah onOur study suggests an early role of the peripheral immune system and the gut in LRRK2 PD and provides a novel model to study early therapeutic immune targets and biomarkers.
Standard operating procedure (SOP) for systemic administration of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) in non-human primates
By savannah onThis protocol details standard operating procedure (SOP) for systemic administration of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) in non-human primates.
Preparation of LRRK1 RCKW cryo-EM grids
By savannah onProtocol used to create LRRK1 RCKW grids for cryo-EM used in Snead, Matyszewski, Dickey et al.
Regulation of mitophagy by the NSL complex underlies genetic risk for Parkinson’s disease at 16q11.2 and MAPT H1 loci
By savannah onThese results enrich our understanding of physiological events regulating mitophagy and establish a novel pathway for drug targeting in neurodegeneration.
Impaired dopamine release in Parkinson’s disease
By savannah onEvidence indicates that impaired dopamine release can result from disruption to a diverse range of Parkinson’s disease-associated genetic and molecular disturbances, and can be considered as a potential pathophysiological hallmark of Parkinson’s disease.
Unconventional secretion of α-synuclein mediated by palmitoylated DNAJC5 oligomers
By savannah onThe secretion of endogenous α-syn mediated by DNAJC5 is also found in a human neuroblastoma cell line, SH-SY5Y, differentiated into neurons in the presence of retinoic acid, and in human-induced pluripotent stem cell-derived midbrain dopamine neurons. We propose that DNAJC5 forms a palmitoylated oligomer to accommodate and export α-syn.
From Policy to Practice: Tracking an Open Science Funding Initiative
By savannah onBy normalizing the open science and compliance process across funding bodies, we hope to simplify and streamline researcher, institutional, and funder workflows, allowing researchers to focus on science by easily leveraging resources and building upon the work of others.
Analysis of glycosphingolipids from human cerebrospinal fluid
By savannah onThe method uses the fluorescent compound anthranilic acid (2-AA) to label oligosaccharides prior to analysis using normal-phase high-performance liquid chromatography. The labeling procedure is rapid, selective, and easy to perform. With the inclusion of a 2AA-labeled chitotriose calibration standard, it is possible to obtain accurate and reproducible molar quantities of individual GSL species.
Genome-wide screen reveals Rab12 GTPase as a critical activator of Parkinson’s disease-linked LRRK2 kinase
By savannah onPublished: The data supports a model in which Rab12 binding to a new site in the LRRK2 Armadillo domain activates LRRK2 kinase for Rab phosphorylation and could serve as a new therapeutic target for a novel class of LRRK2 inhibitors that do not target the kinase domain. View original preprint.
cDNA clones for expression of MitoTag (OMP25) in mammalian cells
By savannah onPlasmids for expression of OMP25 (MitoTag) in human and mouse cells.
cDNA clones for expression of LysoTag (TMEM192) in mammalian cells
By savannah onPlasmids for expression of TMEM192 (LysoTag) in human and mouse cells.
cDNA clones for expression of GolgiTag (TMEM115) in mammalian cells
By savannah onPlasmids for expression of TMEM115 (GolgiTag) in human and mouse cells.
mCh-Miro1(∆TM)-SspB
By savannah onmCh-Miro1(∆TM)-SspB plasmid has a deletion of transmembrane domain and is used for improved light inducible dimer assay.
Glucocerebrosidase, a Parkinson´s disease-associated protein, is imported into mitochondria and regulates complex I assembly and function
By savannah onGlucocerebrosidase, a Parkinson´s disease-associated protein, is imported into mitochondria and regulates complex I assembly and function. Collection of protocols for paper: "Glucocerebrosidase, a Parkinson´s disease-associated protein, is imported into mitochondria and regulates complex I assembly and function."
Cryosectioning mouse brain
By savannah onThis protocol details the cryosectioning of the mouse brain.
Plasmid-reprogramming of human iPSCs
By savannah onThis protocol details about plasmid-reprogramming of human fibroblasts.