Standard Operating Procedure: Mouse transcardiac perfusion protocol
By onMouse transcardiac perfusion protocol and storage.
Automated Immunohistochemistry Staining
By onThe protocols describe the steps for automated immunohistochemistry staining using the Ventana Discovery® XT Immunostainer.
iPS Cell line CRICKi011-A (iFCI016), c.G152A mutation in Exon 3 of SNCA
By onMutations in the SNCA gene cause rare autosomal dominant Parkinson’s disease. iPSC lines were created from individuals with SNCA G51D mutations, showing potential for disease modeling and therapy development for synucleinopathies.
In vitro kinase assay: phosphorylation of PI3Kc1 by TBK1and ULK1
By onProtocol describing in vitro kinase assay, in which PI3Kc is tested for phosphorylation by TBK1 and ULK1 complex either by western blotting (S29 on ATG14L subunit) or mass spectrometry analysis.
H9 ES AAVS1-NGN2 FAM134A-/-; PiggyBac-Keima-RAMP4
By onES cells were modified to create iNeurons lacking FAM134A and expressing Keima-RAMP4 ER-phagy flux reporter. CRISPR/Cas9 was used to introduce NEUROG2 construct in AAVS1 locus. Transfected with Keima-RAMP4 vector.
Temporal landscape of mitochondrial proteostasis governed by the UPRmt
By onThe authors developed a functional proteomics framework, termed MitoPQ (Mitochondrial Proteostasis Quantification), to dissect the UPRmts role in maintaining proteostasis during stress.
Transcriptional analysis of peripheral memory T cells reveals Parkinson’s disease-specific gene signatures–Gene Validation FCS
By onFCS files corresponding to the gene validation experiment described in "Transcriptional analysis of peripheral memory T cells reveals Parkinson’s disease-specific gene signatures"
Preparation of mouse embryonic fibroblast (MEF) feeder plates for hPSC cultures
By onThis protocol describes the preparation of mouse embryonic fibroblasts (MEFs) as feeder cells for human pluripotent stem cell (hPSC) culture.
Immunofluorescence staining for postmortem mouse brain tissue
By onThis is a basic protocol for staining mouse brain tissues using immunofluorescence and immunohistochemistry techniques.
Optogenetic Manipulation (Mouse)
By onThis protocol describes the steps for in vivo ontogenetic manipulation in mice, including assembly of fiber-ferrules, surgical implantation of fibers, and testing procedure.
cDNA clone for bacterial expression of human PPM1H H153D
By onPlasmid for bacterial expression of human PPM1H (H153D); contains TEV protease site for removal of 6His tag.
Brain processing, slicing and immunohistochemistry protocol
By onThis protocol is a step by step procedure from collecting brain samples to immunohistochemical staining and mounting brain slices.
Transcriptional analysis of peripheral memory T cells reveals Parkinson’s disease-specific gene signatures–Cell Surface FCS
By onFCS files corresponding to the cell surface experiment described in "Transcriptional analysis of peripheral memory T cells reveals Parkinson’s disease-specific gene signatures"
CRISPR tagging of the EEA1 gene in H9 ES cells for Endo-IP
By onProtocol for tagging EEA1 gene in human H9 ES cells with a 3X flag using CAS9 and oligonucleotide template is described.
The AAA+ chaperone VCP disaggregates Tau fibrils and generates aggregate seeds in a cellular system
By onProtein aggregates, like tau, are associated with neurodegenerative diseases. The authors show that the AAA+ chaperone, VCP, is recruited to ubiquitylated Tau, resulting in disaggregation.
The SATB1-MIR22-GBA axis mediates glucocerebroside accumulation inducing a cellular senescence-like phenotype in dopaminergic neurons
By onStudy shows SATB1, MIR22HG, and GBA genes form a pathway in Parkinson's Disease. Dysregulation leads to GluCer accumulation, causing cellular senescence in dopaminergic neurons, potentially explaining neuroinflammation seen in PD and aging.
Pole Test to assess motor coordination in parkinsonian mice
By onThe pole test evaluates the motor coordination ability of a mouse by having them grasp a pole and then descend to its home cage.
Mouse Ovariectomy
By onThis protocol describes the steps to perform an ovariectomy in a mouse. This procedure is used to induce menopause.
