Simulations predict differing phase responses to excitation vs. inhibition intheta-resonant pyramidal neurons
By onThis research highlights how cortical neurons respond differently to oscillatory inputs, impacting neuronal responses to network state shifts.
Expression and purification of PI3KC3-C1 complex
By onProtocol describing expression and purification of PI3KC3-C1 complex.
TBK1 knockdown and rescue in Hela-M cells
By onThis protocol transiently depletes TBK1 from HeLa cells and re-introduces a tagged TBK1 along with other relevant components of mitophagy.
Synapsin E-domain is essential for α-synuclein function
By onSynucleins and synapsins are thought to cooperate to regulate synaptic vesicle recycling, but the mechanism is not known. Here we identify the synapsin E-domain as an essential binding partner of α-syn, enabling the function of α-syn at the synapse.
Genome-wide determinants of mortality and clinical progression in Parkinson’s disease – Summary statistics
By onSummary statistics from "Genome-wide determinants of mortality and clinical progression in Parkinson’s disease."
Primary data associated with the manuscript “Genome-wide screen reveals Rab12 GTPase as 2 a critical activator of Parkinson’s disease-linked LRRK2 kinase” (3/3)
By onPrimary data associated with the manuscript, "Genome-wide screen reveals Rab12 GTPase as 2 a critical activator of Parkinson’s disease-linked LRRK2 kinase."
AAVS1 Knock-in
By onThis protocol describes the standard procedure to knock-in constructs to the AAVS1 safe harbor locus in hPSCs.
Intracellular neuromelanin quantification
By onProtocol for intracellular neuromelanin quantification.
Neuromelanin accumulation drives endogenous synucleinopathy in non-human primates
By onA new NHP PD model was developed by inducing neuromelanin (NM) accumulation in dopaminergic neurons, leading to synucleinopathy and neuron degeneration similar to human PD neuropathology. Lowering NM levels may offer PD therapeutic strategies.
NAP1-mCherry
By onThis protocol describes how to express and purify human NAP1 tagged C-terminally with mCherry.
Crystal structure ATG9 HDIR in complex with the ATG13:ATG101 HORMA dimer
By onCrystal structure ATG9 HDIR in complex with the ATG13:ATG101 HORMA dimer (Method: X-RAY DIFFRACTION Resolution: 2.41 Å R-Value Free: 0.251 R-Value Work: 0.204 R-Value Observed: 0.205)
LRRK2 regulates the activation of the unfolded protein response and antigen presentation in macrophages during inflammation
By onAbsence of PINK1 leads to MitAP over-activation engaging autoimmune mechanisms. This pathway is induced by TLR4, cGAS-STING, and UPR activation in response to inflammatory signals. LRRK2 and STING regulate transition from innate to adaptive immunity
Protocol for hippocampal neuronal cultures
By onThis protocol details the procedue for preparation of neuronal culturs from mice hippocampi as it was performed in Guillén-Samander et al. 2021 article "VPS13D bridges the ER to mitochondria and peroxisomes via Miro" but can also be used to prepare culturs of cortical neurons.
Genome-wide determinants of mortality and motor progression in Parkinson’s disease
By onThe authors examined the impact of gene variants on mortality and cognitive impairment in PD. Only the non-Gaucher disease causing GBA PD risk variant E326K, of the known PD risk variants, was associated with progression in PD.
Glucocerebrosidase is imported into mitochondria and preserves complex I integrity and energy metabolism – ASAP protocol collection
By onCollection of protocols of Deleidi Lab used in the publication: "Glucocerebrosidase is imported into mitochondria and preserves complex I integrity and energy metabolism."
Central and Peripheral Inflammation: Connecting the Immune Responses of Parkinson’s Disease
By onAuthors highlight the important work being done that implicates central and peripheral inflammation in playing a role in PD.
