Pole Test to assess motor coordination in parkinsonian mice
By onThe pole test evaluates the motor coordination ability of a mouse by having them grasp a pole and then descend to its home cage.
Mouse Ovariectomy
By onThis protocol describes the steps to perform an ovariectomy in a mouse. This procedure is used to induce menopause.
Motor learning selectively strengthens cortical and striatal synapses of motor engram neurons
By onIn this study, Hwang, Roth, et al. find that motor learning recruits a population of engram neurons in the motor cortex that are reactivated during task performance. Motor learning leads to selective remodeling of dendritic spines and strengthening of outputs to the striatum of M1 engram neurons.
Border-associated macrophages mediate the neuroinflammatory response in an alpha-synuclein model of Parkinson disease
By onBorder-associated macrophages (BAMs) are crucial in Parkinson's disease pathogenesis by initiating neuroinflammation, highlighting a potential target for therapeutic intervention.
Mouse reaching task
By onThe repository includes materials for building and analyzing data from a mouse-reaching task: a bill of materials, 3D models, schematics, an Arduino controller, video recording scripts, and data analysis scripts using DLC.
iPS Cell line: CRICKi012-A (iFCI017), c.G152A mutation in Exon 3 of SNCA
By onMutations in SNCA gene cause rare Parkinson’s disease. iPSC lines from individuals with SNCA G51D mutation were generated successfully, showing normal characteristics. These iPSC lines can aid in studying synucleinopathies for potential therapies.
CD3 Cell Density in Substantia Nigra and Cerebral Peduncle Image Analysis
By onThis protocol describes how to measure CD3 density in the substantia nigra and cerebral peduncle using haematoxylin and DAB-stained brain sections.
Yeast cells live fluorescence imaging
By onProtocol for sample preparation and live fluorescence imaging of yeast cells.
Preparing samples for NGS
By onThis protocol describes a standard procedure used to prepare PCR samples for Next Generation Sequencing (NGS).
Unaltered T cell responses to common antigens in individuals with Parkinson’s disease
By onT cells have been shown to be overactive in individuals with PD. The authors tested a wide variety of commonly encountered immune targets on PD and non-PD control derived T cells and observed no differences between their immune responses.
Mitochondrial Antigen Presentation in RAW macrophages
By onThis protocol details methods for 3-day Mitochondrial Antigen Presentation Assay in a murine macrophage cell line (RAW) that expresses a glycoprotein B (gB) of herpes simplex virus 1 (HSV1) targeted to the mitochondrial matrix (mito-gB). A gB-specific CD8+ T cell hybridoma recognizing the gB498–505peptide loaded on MHC class I molecules is also used to monitor antigen presentation through a beta-galactosidase assay kit.
Early Endosome Capture Proteomics and its Application to Amyloid Precursor Protein Intramembrane Processing by β and γ-Secretases
By onDegradation and recycling of plasma membrane proteins occur through the endolysosomal system. Endo-IP, captures early endosomes for proteomic analysis, revealing insights into protein trafficking and disease-related cargo like APP in Alzheimer's.
Glucocerebrosidase 1 and leucine-rich repeat kinase 2 in Parkinson disease and interplay between the two genes
By onReview: This review focuses on the endolysosomal pathway roles of GBA and LRRK2, highlighting the role and activity of Rab GTPases as LRRK2 substrates.
(x6) GBA heterozygous and homozygous mutant fibroblast lines available to share
By onCell lines deposited at ATCC used in a publication (10.1093/hmg/ddac233) include UCL-CTRL001, UCL-CTRL002, UCL-CTRL003, UCL-YCTRL001, UCL-E001K, UCL-N001S, and UCL-N002S, each with specific RRIDs.
shoebridges/asap_phosphosites: Preview Release
By onSource data associate with publication "Unconventional Initiation of PINK1/Parkin Mitophagy by Optineurin"
iPSC differentiation into Microglia
By onThis protocol describes iPSC differentiation into microglia.
