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ENS Quantification
Output Details
Labelled enteric neurons in living mice with recombinant adeno-associated viruses (rAAVs) expressing fluorescent proteins, and used tissue clearing techniques to enhance visualization of intact GI tissue.
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Meet the Authors
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Bryan B. Yoo
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Jessica A. Griffiths
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Peter Thuy-Boun
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Kelly Weldon
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Collin Challis
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Michael J. Sweredoski
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Taren M. Thron
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Gil Sharon
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Annie Moradian
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Gregory Humphrey
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Qiyun Zhu
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Justin Shaffer
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Dennis W. Wolan
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Pieter C. Dorrestein
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Rob Knight
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Viviana Gradinaru, PhD
Lead PI (Core Leadership): Team Gradinaru,
California Institute of Technology
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Sarkis Mazmanian
Co-PI (Core Leadership): Team Sulzer, Team Gradinaru,
California Institute of Technology
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Mouse Stereotaxic Surgery-Arber Lab
This protocol describes the steps for performing stereotaxic surgery in mice. It is applicable to intracranial injections (e.g. virus, drug) and placement of implants (e.g. optical fibers, electrode arrays) into targeted regions of mouse brains.