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A fluorescence-based in vitro scrambling assay for yeast MCP1 and human XK
Output Details
Description
VPS13 proteins are proposed to function at contact sites between organelles as bridges for lipids to move directionally and in bulk between organellar membranes. VPS13s are found to interact with integral membrane proteins, like MCP1 in yeast or XK in humans. We showed that MCP1 and XK scramble phospholipids in vitro. Here I describe the detailed procedure of purification, reconstitution, and scrambling assay for both MCP1 and XK.
Identifier (DOI)
10.17504/protocols.io.kxygxzyqdv8j/v1