Purification and analysis of SKP1-FBXO7 complexes
By onProtocol for the biochemical purification and analysis of SKP1-FBXO7 complexes.
Preparation of LRRK2 RCKW cryo-EM grids
By onThis is Leschziner's Lab updated protocol for making cryo-EM grids for LRRK2 RCKW. This protocol, when using lower protein concentration, results in better monomer and dimer formation than the old protocol.
Fixation of HeLa-M cells expressing Halo and SNAP fusion proteins conjugated to ligands
By onProtocol describing fixation of HeLa-M cells expressing Halo and SNAP fusion proteins conjugated to ligands.
Soluble and insoluble A-SYN fractionation
By onSoluble/insoluble alpha-synuclein fractionation is a technique used to separate different forms of the alpha-synuclein protein based on their solubility properties.
HEK293 cell culture for co-immunoprecipitation experiments
By onProtocol describing how to perform HEK293 cell culture for the purpose of co-immunoprecipitation experiments.
pBPK1520-HEK3-CTTins-ng
By onIt can be used to introduce HEK3 CTT insertion using prime editing, PE3 approach. It serves as a good positive control for prime editing.
iNeuron differentiation from human iPSCs
By onThe authors adapted a previously-described method for differentiating iPSCs stably expressing mNGN2 at a safe-harbor locus into human excitatory glutamatergic neurons.
Determination of free and protein-bound DA and NE and their metabolites and oxidation products by UPLC-MS/MS method
By onProtocol for the determination of free and protein-bound DA and NE and their metabolites and oxidation products by UPLC-MS/MS method.
Endogenous tagging of the YIPF4 gene with mNEON Green and imaging of these cells by microscopy
By onProtocol for tagging YIPF4 gene with mNEON in cells using CRISPR. Method involves editing N-terminus of YIPF4 in WT and FIP200-/- HEK293T cells. Imaging mNEON-YIPF4 in Golgi with GOLGB1 marker is also detailed.
powereQTL – An R shiny application for calculating sample size and power of bulk tissue and single-cell eQTL analysis
By onEasy-to-use web application for calculating sample size and power of bulk tissue and single-cell eQTL analysis
Confocal imaging on Nikon AXR confocal microscope
By onA step by step protocol to acquire images on Nikon AXR confocal set-up.
Mito and Glycolysis Stress Tests for Enteroendocrine Cells – Hutu-80
By onMito and glycolysis stress tests for enteroendocrine cells - Hutu-80
Organelle isolation from mouse tissues expressing organelle tags
By onThe organelles purified using this method are highly enriched, intact, largely contaminant-free and can be used for various downstream applications, including immunoblotting analysis and proteomic analysis, but also lipidomic or metabolomic analysis.
Image processing to investigate mitophagy in HelaM and neurons
By onThis is a method for imaging cells and quantifying subcellular structures in the resulting data. We developed this protocol for assessing clearance of mitochondria involving OPTN and TBK1, however the method could be used for other applications.
Microfluidic Chip Production v1.1 V.2
By onProtocol for producing microfluidic chips used in HyDrop experiment. Visit for more info
