Expansion of mouse embryonic fibroblasts (MEFs) for hPSC cultures
By onThis protocol describes the expansion of mouse embryonic fibroblasts (MEFs) as feeder cells for human pluripotent stem cell (hPSC) culture.
AAVS1-SA-neo-CAGGS-PE2-2A-GFP
By onIt can be used to generate knock-in cell line faciliating prime editing.
Subcellular proteomics of dopamine neurons in the mouse brain
By onUnderstanding the proteome of dopamine neuron is difficult due to the complex cytoarchitecture of the neurons. The authors were able to map the somatodendritic and axonal proteomes of midbrain dopaminergic neurons.
Cell culture, transfection, immunocytochemistry, and imaging
By onThis protocol is to help with the maintenance, transfection, immunocytochemistry, and imaging of adherent mammalian cells
Macros and pipelines for analysis of image analysis
By onImageJ macros and pipelines for analysis of pUb puncta, Keima Ratio, p62nuclei and Parkin recruitment to mitochondria.
Basal ganglia neurons in healthy and parkinsonian primates generate recurring sequences of spikes
By onBasal ganglia neurons fire in recognizable, repeating sequences of interspike intervals (ISIs), the incidence of which is influenced by the induction of Parkinsonism.
Rodent models based on endolysosomal genes involved in PArkinson’s disease
By onGenes linked to endolysosomal function are connected to Parkinson's disease, suggesting a role in its development. Rodent models targeting these genes like LRRK2 and GBA1 show parkinsonian features, aiding in understanding and treating the disease.
Genetic and pharmacological reduction of CDK14 mitigates synucleinopathy
By onDecreasing alpha-synuclein levels is a potential therapeutic approach for synucleinopathies. The authors identified CDK14 regulates alpha-synuclein and show reduction of CDK14 reduces alpha-synuclein and PD-like characteristics.
Purification and analysis of SKP1-FBXO7 complexes
By onProtocol for the biochemical purification and analysis of SKP1-FBXO7 complexes.
Preparation of LRRK2 RCKW cryo-EM grids
By onThis is Leschziner's Lab updated protocol for making cryo-EM grids for LRRK2 RCKW. This protocol, when using lower protein concentration, results in better monomer and dimer formation than the old protocol.
Fixation of HeLa-M cells expressing Halo and SNAP fusion proteins conjugated to ligands
By onProtocol describing fixation of HeLa-M cells expressing Halo and SNAP fusion proteins conjugated to ligands.
Soluble and insoluble A-SYN fractionation
By onSoluble/insoluble alpha-synuclein fractionation is a technique used to separate different forms of the alpha-synuclein protein based on their solubility properties.
HEK293 cell culture for co-immunoprecipitation experiments
By onProtocol describing how to perform HEK293 cell culture for the purpose of co-immunoprecipitation experiments.
pBPK1520-HEK3-CTTins-ng
By onIt can be used to introduce HEK3 CTT insertion using prime editing, PE3 approach. It serves as a good positive control for prime editing.
