Synaptic Location Is a Determinant of the Detrimental Effects of α-Synuclein Pathology to Glutamatergic Transmission in the Basolateral Amygdala
By onStudy explores α-synuclein's impact on amygdala in Parkinson's. Aggregation affects cortico-BLA transmission, linked to psychiatric deficits in PD.
Passaging of feeder-free hPSCs
By onThis protocol describes the process of passaging human pluripotent stem cells (hPSCs) for feeder-free culturing of hPSCs using Accutase or ReLeSF.
fMRI tasks to explore emotion-motor interactions
By onCode for fMRI tasks designed to explore emotion-motor interactions—Active Escape and Approach-Avoidance tasks in the Socal Kinesia and Incentivization for Parkinson's Disease (SKIP) dataset.
Cx3cr1-cre;Ercc1-/fl mice
By onMicroglia may experience higher levels of DNA damage and potential senescence due to internal factors.
Assay for Dual Rab GTPase binding to the LRRK2 Armadillo Domain
By onThe LRRK2 Armadillo domain contains multiple Rab GTPase binding sites. This assay shows that the sites can be occupied simultaneously.
pCAG-GST-ATG101-W110D/F112D
By onPlasmid: Expresses human ATG101 HORMA W110D/F112D mutant in mammalian cells.
CURTAIN—A unique web-based tool for exploration and sharing of MS-based proteomics data
By onLinks can also be reported in publications allowing readers to further survey the reported data. The authors discuss benefits for the research community of publishing proteomic data containing a shareable web-link.
Regulatory imbalance between LRRK2 kinase, PPM1H phosphatase, and Arf6 GTPase disrupts the axonal transport of autophagosomes
By onThe authors found that LRRK2-hyperphosphorylated RABs disrupt the axonal transport of autophagosomes by perturbing the coordinated regulation of cytoplasmic dynein and kinesin motors.
Data set of the manuscript “Neuronal hyperactivity-induced oxidant stress promotes in vivo a-synuclein brain spreading”
By onData set of the manuscript "Neuronal hyperactivity-induced oxidant stress promotes in vivo a-synuclein brain spreading."
Preparing feeder-free hPSCs for nucleofection
By onThis protocol describes the standard procedure preparing feeder-free human pluripotent stem cells (hPSCs) for nucleofection.
H9 ES AAVS1-NGN2 TEX264-/-; PiggyBac-Keima-REEP5
By onES cells modified with CRISPR/Cas9 to lack TEX264, express Keima-REEP5 ER-phagy flux reporter, NEUROG2, and mKeima fluorescent protein. Derived from human embryonic stem cells at the blastocyst stage.
H9 ES AAVS1-NGN2 FLAG-EEA1::TMEM192-HA
By onES cells were modified to produce iNeurons expressing FLAG-tagged EEA1 and TMEM192-HA. NEUROG2 construct was inserted into AAVS1 locus using CRISPR/Cas9. EEA1 was N-terminally tagged with a 3xFLAG tag.
Western blot protocol for detecting ATP10B in mouse/rat brain
By onProtocol for detection of ATP10B in rat and mouse brain tissue by Western blotting.
Evaluating endocytic rate in cells
By onAssess endocytic rate in cells using tagged transferrin (Alexa647) and flow cytometry readout.
Sample preparation for TMT-based total and phospho-proteomic analysis of cells and tissues
By onMass spectrometry-based proteomics and phosphoproteomics are highly sensitive and unbiased techniques to study the proteome and phosphoproteome at a global scale.
Modelling human brain-wide pigmentation in rodents recapitulates age-related multisystem neurodegenerative deficits
By onAuthors describe an animal model of human-like neuromelanin pigmentation. The animals display age-related neuronal dysfunction and degeneration affecting numerous brain circuits and body tissues.
CRISPR-Cas9 screen in NIH-3T3 cells to identify modulators of LRRK2 function
By onThis protocol describes a pooled, CRISPR Cas9 screen to identify modulators of LRRK2 activity.
