Dopaminergic denervation and associated MRI microstructural changes in the nigrostriatal projection in early Parkinson’s disease patients
By Emma Sherrell onThe asymmetry between striatal and SNc changes for both dopaminergic depletion and microstructural degeneration biomarkers are consistent with a neurodegenerative process that begins in the striatal terminals before progressing toward the cell bodies in the SNc.
Single-cell somatic copy number variants in brain using different amplification methods and reference genomes
By Emma Sherrell onThis study demonstrates that the authors' semi-automated protocol is suitable for shotgun metagenomic analysis, by significantly producing higher DNA fragment sizes while allowing for improved sample treatment logistics with reduced technical variability and without compromising the structure of the oral microbiome.
Testing AAVrg-hSyn-mCherry in the Putamen of Rhesus Macaques
By Emma Sherrell onViral Vector: Two rhesus macaque animals were tested for AAVrg-hSyn-mCherry in the putamen. The authors observed abundant mCherry-positive neurons in expected targets (i.e., motor cortical regions known to project to the putamen).
Immunofluorescent labeling of cultured cells
By savannah onThis protocol describes the process of Paraformaldehyde fixation and immunofluorescent-labeling of cultured cells.
Midbrain viral injections for striatal fiber photometry in mice
By savannah onWhile this protocol is focused on injections of AAV viruses into the midbrain, it can be easily modified to inject into other brain regions or other viruses.
pCMV-pOTC-GFP
By savannah onExpression of GFP fused with pOTC, a leader peptide from mitochondrial matrix enzyme ornithine transcarbamylase.
pCMV-XPACK-DNAJC5 (L115R)- HaloTag
By savannah onMammalian expression of XPACK-DNAJC5 (L115R)-HaloTag.
