3D FIBSEM CLEM
By savannah onThis protocol details the procedure of Correlative Light Microscopy and Electron Microscopy (CLEM) with 3D Focus Ion Beam Scanning Electron Microscopy (FIBSEM) technique in Zeiss Crossbeam 550 FIBSEM system.
Inhibition of striatal dopamine release by the L-type calcium channel inhibitor isradipine co-varies with risk factors for Parkinson’s
By savannah onPublished: This data show that LTCC function in DA axons, and isradipine effect, are locally governed and suggest they vary in a manner that in turn might impact on, or reflect, the cellular stress that leads to parkinsonian degeneration. View original preprint.
Synaptic location is a determinant of the detrimental effects of α-synuclein pathology to glutamatergic transmission in the basolateral amygdala
By savannah onαSyn expression is restricted in a subset of glutamatergic synapses in BLA and its aggregation decreases cortico-BLA transmission through both gained toxicity and loss of normal function. These results might be relevant to the reduced cortical control of amygdala function that has been associated with psychiatric deficits in PD.
Vibrational stabilization of cluster synchronization in oscillator networks
By savannah onPreprint: Deviations from normal cluster synchronization patterns are closely associated with various malfunctions, such as neurological disorders in the brain. Here, the authors employ an open-loop control strategy, vibrational control, which does not require any state measurements. Additionally, they conduct numerical experiments to demonstrate their theoretical findings.
Sample preparation for TMT-based total and phospho-proteomic analysis of cells and tissues
By savannah onMass spectrometry-based proteomics and phosphoproteomics are highly sensitive and un-biased techniques to study the proteome and phosphoproteome at a global scale. Using our protocols, we routinely identify and quantify >35,000 phosphosites and ~10,000 protein groups.
Localization of PPM1H phosphatase tunes Parkinson’s disease-linked LRRK2 kinase-mediated Rab GTPase phosphorylation and ciliogenesis
By savannah onPreprint: The data support a model in which localization drives PPM1H substrate selection and centriolar PPM1H is critical for regulation of Rab GTPase-regulated ciliogenesis. Moreover, Golgi localized PPM1H maintains active Rab GTPases on the Golgi to carry out their non-ciliogenesis-related functions in membrane trafficking.
Immunohistochemistry of Human Brain Striatum: Ciliation of cholinergic neurons and astrocytes
By savannah onThe authors present a method to identify the same brain region in human postmortem tissue to evaluate ciliary status. The striatum is identified morphologically and also by immunofluorescence microscopy using anti-DARPP-32 antibodies.
CURTAIN – A Unique web-based tool for exploration and sharing of MS-based proteomics data
By savannah onPublished: Links can also be reported in publications allowing readers to further survey the reported data. The authors discuss benefits for the research community of publishing proteomic data containing a shareable web-link. View original preprint.
Sample collection and processing for RNA analysis
By savannah onThis protocol delineates the steps taken to collect frozen postmortem human brain samples and process them for RNA extraction and analysis.
Structure of human ULK1 complex core (2:1:1 stoichiometry)
By savannah onOrganism(s): Homo sapiens Expression System: Homo sapiens Mutation(s): No Deposited: 2023-04-28 Released: 2023-06-21 Deposition Author(s): Chen, M., Hurley, J.H. Funding Organization(s): National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS), Michael J. Fox Foundation
LRRK2 regulates the activation of the unfolded protein response and antigen presentation in macrophages during inflammation
By savannah onRemarkably, the PD-related protein LRRK2 acted with STING upstream of the UPR to regulate the transition from innate to adaptive immunity, thereby identifying this PD-related protein as a key player in the immune response during inflammation.
Characterizing the diversity of enteric neurons using Dopamine Transporter (DAT)-Cre reporter mice
By savannah onGiven the clear heterogeneity of DA gut neurons, further investigation is warranted to define their functional signatures and discover their inherent biological differences that put these cells at risk for neurodegeneration.
Structure of human PI3KC3-C1 complex
By savannah onOrganism(s): Homo sapiens Expression System: Homo sapiens Mutation(s): No Deposited: 2023-04-29 Released: 2023-06-21 Deposition Author(s): Chen, M., Hurley, J.H. Funding Organization(s): National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS), Michael J. Fox Foundation
Structure of human ULK1C:PI3KC3-C1 supercomplex
By savannah onOrganism(s): Homo sapiens Expression System: Homo sapiens Mutation(s): No Deposited: 2023-05-05 Released: 2023-06-21 Deposition Author(s): Chen, M., Hurley, J.H. Funding Organization(s): National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS), Michael J. Fox Foundation
Structure of human ULK1 complex core (2:2:2 stoichiometry) in the PI3KC3-C1 mixture
By savannah onOrganism(s): Homo sapiens Expression System: Homo sapiens Mutation(s): No Deposited: 2023-05-04 Released: 2023-06-21 Deposition Author(s): Chen, M., Hurley, J.H. Funding Organization(s): National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS), Michael J. Fox Foundation
Structure of human ULK1 complex core (2:2:2 stoichiometry) of the ATG13(450-517) mutant
By savannah onOrganism(s): Homo sapiens Expression System: Homo sapiens Mutation(s): No Deposited: 2023-05-05 Released: 2023-06-21 Deposition Author(s): Chen, M., Hurley, J.H. Funding Organization(s): National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS), Michael J. Fox Foundation
Microscopy-based evaluation of mtKeima flux in hESC-derived Ctrl and FBXO7-/- iNeurons
By savannah onPARK15/FBXO7 is dispensable for PINK1/Parkin mitophagy in iNeurons and HeLa cell systems. https://www.embopress.org/doi/full/10.15252/embr.202256399
PARK15/FBXO7 is dispensable for PINK1/Parkin mitophagy in iNeurons and HeLa cell systems
By savannah onGlobal proteomics of neurogenesis in the absence of FBXO7 reveals no obvious alterations in mitochondria or other organelles. These results argue against a general role for FBXO7 in Parkin-dependent mitophagy and point to the need for additional studies to define how FBXO7 mutations promote parkinsonian–pyramidal syndrome.
Purification and analysis of SKP1-FBXO7 complexes
By savannah onProtocol for the biochemical purification and analysis of SKP1-FBXO7 complexes.
Confirming circRNA expression by qPCR
By savannah onThis protocol delineates a qPCR method to confirm the expression of circRNAs extracted from brain samples.