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Surgical procedures for simultaneous optogenetic manipulation in the SNc and acute fiber photometry in the dorsal striatum
Output Details
Description
This protocol describes the three surgical procedures required for simultaneous optogenetic stimulation in the Substantia Nigra pars compacta (SNc) and acute fiber photometry in the dorsal striatum:
1. Viral injection of a red-shifted channelrhodopsin, ChRmine, in the SNc, and a green dopamine sensor, GRAB-DA3m, in the dorsal striatum.
2. Fiber-optic cannula implantation into the SNc for optogenetic stimulation.
3. Craniotomy above the dorsal striatum for acute fiber photometry.
Viral injection for ChRmine and GRAB-DA
Identifier (DOI)
10.17504/protocols.io.e6nvwd6qdlmk/v1