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  • Structural basis for the specificity of PPM1H phosphatase for Rab GTPases

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    Raw data associated with DOI: 10.15252/embr.202152675

  • LC3-lipidation-assay

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    Protocol for an in vitro LC3 lipidation assay using purified proteins and synthetic liposomes.

  • Membrane Tube Image Analysis

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    This protocol details Membrane Tube Image Analysis.

  • Bile acids and neurological disease

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    This review will focus on how bile acids are being used in clinical trials to treat neurological diseases due to their central involvement with the gut-liver-brain axis and their physiological and pathophysiological roles in both normal brain function and multiple neurological diseases. The synthesis of primary and secondary bile acids species and how the regulation of the bile acid pool may differ between the gut and brain is discussed. The expression of several bile acid receptors in brain and their currently known functions along with the tools available to manipulate them pharmacologically are examined, together with discussion of the interaction of bile acids with the gut microbiome and their lesser-known effects upon brain glucose and lipid metabolism. How dysregulation of the gut microbiome, aging and sex differences may lead to disruption of bile acid signalling and possible causal roles in a number of neurological disorders are also considered. Finally, we discuss how pharmacological treatments targeting bile acid receptors are currently being tested in an array of clinical trials for several different neurodegenerative diseases

  • Hippocampal Neuronal Culture

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    This protocol describes the procedure for hippocampal neuronal cultures from new - born mouse pups.

  • pCMV-DNAJC5-ΔJ (Δ14–82)

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    Mammalian expression of DNAJC5-ΔJ (Δ14-82)

  • Western blot – alpha-synuclein

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    This protocol describes how to detect alpha-synuclein in protein derived from STC-1 cells by western blot using DAB/peroxidase or ECL to visualize the bands.

  • Oligo2-cre;Ercc1-/fl mice

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    Oligodendrocytes may experience higher levels of DNA damage and potential senescence due to internal factors.

  • Creating pooled CRISPR-Cas9 knock-outs in NIH-3T3 cells

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    To validate a genome wide CRISPR screen, the authors select the top hits and create lentiviruses to validate the hits.

  • Single-molecule Immunofluorescence Tissue Staining Protocol for Oligomer Imaging V.3

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    This protocol details background fluorescence quenching and immunofluorescence staining of human brain tissue for oligomer imaging.

  • Protocols for “Characterizing the diversity of enteric neurons using Dopamine Transporter (DAT)-Cre reporter mice”.

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    This is a collection of protocols associated to the manuscript "Characterizing the diversity of enteric neurons using Dopamine Transporter (DAT)-Cre reporter mice".

  • pU6-pegRNA-LRRK2-G2019S-3a

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    It can be used to introduce LRRK2-G2019S mutation using prime editing.

  • Immunochemistry on paraffin sections

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    Protocol for immunochemistry on paraffin sections.

  • Sample Collection and Processing for RNA Analysis

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    This protocol delineates the steps taken to collect frozen postmortem human brain samples and process them for RNA extraction and analysis.

  • The emerging role of LRRK2 in tauopathies

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    Parkinson’s disease (PD) is conventionally described as an α-synuclein aggregation disorder, defined by Lewy bodies and neurites, and mutations in leucine-rich repeat kinase 2 (LRRK2) are the most common autosomal dominant cause of PD. However, LRRK2 mutations may be associated with diverse pathologies in patients with Parkinson’s syndrome including tau pathology resembling progressive supranuclear palsy (PSP). The recent discovery that variation at the LRRK2 locus is associated with the progression of PSP highlights the potential importance of LRRK2 in tauopathies. Here, the authors review the emerging evidence and discuss the potential impact of LRRK2 dysfunction on tau aggregation, lysosomal function, and endocytosis and exocytosis.

  • H9 ES AAVS1-NGN2 FAM134C-/-

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    ES cells manipulated with CRISPR/Cas9 lack FAM134C receptor for ER-phagy. NEUROG2 construct introduced via AAVS1 locus in human embryonic stem cells, creating iNeurons. Derived from blastocyst stage, female, Homo sapiens.

  • Challenging Beam Test

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    The challenging beam test is used to measure motor performance, coordination, and balance.

  • pBMN HA-ATG13

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    Plasmid: For expression of ATG13(Δ1-198) tagged with HA.

  • Mouse Brain Tissue Collection and Analysis

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    This protocol describes the dissection and collection of coronal sections of the striatum and midbrain from a mouse brain. The tissue can be used in a number of applications and here we describe two: the measurement catecholamine levels using high-performance liquid chromatography (aided by a neurochemistry core) and the distribution of the vesicular monoamine transporter (VMAT2) using a radioligand binding assay.

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