Calculating mitochondrial protein solubility
By Emma Sherrell onProtocol for generating solubility calculations for mitochondrial proteins from starting 'soluble' and 'insoluble' fraction mass spectrometry data, using MaxQuant and Perseus software pipelines.
Reverse-phase high pH fractionation (using Thermo Fisher, Cat# 84868)
By Emma Sherrell onThis protocol uses the Pierce™ High pH Reversed-Phase Peptide Fractionation Kit (Thermo Fisher, Cat# 84868).
Preparation of soluble and insoluble mitochondrial protein fractions for immunoblotting
By Emma Sherrell onPreparation of soluble and insoluble mitochondrial protein fractions from HeLa cells for immunoblotting.
Mitochondrial isolation from HeLa cells
By Emma Sherrell onMitochondrial isolation and quantification from HeLa cells.
Western blotting using the BioRad Criterion Blotter system
By Emma Sherrell onProtocol for performing an SDS-PAGE Western blot analysis using the BioRad Criterion Blotter system.
Preparing whole cell samples for immunoblot analysis
By Emma Sherrell onProtocol for preparation of HeLa cell lysates for immunoblot analysis.
Induction of starvation stress
By Emma Sherrell onInduction of starvation stress using EBSS in HeLa cells.
Mitophagy induction using Difereprone
By Emma Sherrell onMitophagy induction in HeLa cells using Difereprone (DFP).
Mitophagy induction using Oligomycin/Antimycin A
By Emma Sherrell onMitophagy induction in HeLa cells using Oligomycin/Antimycin A.
Generation of stable cell lines using viral infection
By Emma Sherrell onProtocol for generation and precipitation of retrovirus, and infection of HeLa cells to generate stable cell lines.
Reduction of a-synuclein aggregates by PIKfyve inhibition via TFEB-mediated lysosomal biogenesis in a Parkinson’s disease model
By Emma Sherrell onPreprint: In the present study, the authors exploited the SH-SY5Y cell model overexpressing a pro-aggregation form of alpha-synuclein to investigate the efficacy of PIKfyve-mediated lysosomal biogenesis, through TFEB, as a potential target for Parkinson's therapy.
Feacal metagenomic data related to “Evaluation of an Adapted Semi-Automated DNA Extraction for Human Salivary Shotgun Metagenomics”
By Emma Sherrell onThis study demonstrates that the authors' semi-automated protocol is suitable for shotgun metagenomic analysis, by significantly producing higher DNA fragment sizes while allowing for improved sample treatment logistics with reduced technical variability and without compromising the structure of the oral microbiome.
Salivary collection participant instructions using OMNIgene ORAL OM 501 device
By Emma Sherrell onSalivary collection participant instructions using OMNIgene ORAL OM 501 device.
R scripts for analysis of publication “Sex distribution of GBA1 Variants Carriers with Dementia with Lewy Bodies and Parkinson Disease”
By Emma Sherrell onR scripts for analysis of publication "Sex distribution of GBA1 variant carriers with dementia with Lewy Bodies and Parkinson's disease."
Biochemical consequences of glucocerebrosidase 1 mutations in Parkinson’s disease
By Emma Sherrell onPerspective on the biochemical consequences of glucocerebrosidase 1 mutations in Parkinson’s disease.
Lentiviral vector plasmid for overexpression of ATP10B (E993A)
By Emma Sherrell onPlasmid: Lentiviral vector plasmid for overexpression of ATP10B (E993A) validated for use in cell culture.
Lentiviral vector plasmid for overexpression of ATP10B (I1038T)
By Emma Sherrell onPlasmid: Lentiviral vector plasmid for overexpression of ATP10B (I1038T) validated for use in cell culture.
Lentiviral vector plasmid for knock down of ATP10B (miR5 Hs)
By Emma Sherrell onPlasmid: Lentiviral vector plasmid for knock down of ATP10B (miR5, Hs) validated for use in cell culture.
Dopaminergic denervation and associated MRI microstructural changes in the nigrostriatal projection in early Parkinson’s disease patients
By Emma Sherrell onThe asymmetry between striatal and SNc changes for both dopaminergic depletion and microstructural degeneration biomarkers are consistent with a neurodegenerative process that begins in the striatal terminals before progressing toward the cell bodies in the SNc.
