shoebridges/asap_phosphosites: Preview Release
By onSource data associate with publication "Unconventional Initiation of PINK1/Parkin Mitophagy by Optineurin"
Glucocerebrosidase 1 and leucine-rich repeat kinase 2 in Parkinson disease and interplay between the two genes
By onReview: This review focuses on the endolysosomal pathway roles of GBA and LRRK2, highlighting the role and activity of Rab GTPases as LRRK2 substrates.
Mitochondrial Antigen Presentation in RAW macrophages
By onThis protocol details methods for 3-day Mitochondrial Antigen Presentation Assay in a murine macrophage cell line (RAW) that expresses a glycoprotein B (gB) of herpes simplex virus 1 (HSV1) targeted to the mitochondrial matrix (mito-gB). A gB-specific CD8+ T cell hybridoma recognizing the gB498–505peptide loaded on MHC class I molecules is also used to monitor antigen presentation through a beta-galactosidase assay kit.
Early Endosome Capture Proteomics and its Application to Amyloid Precursor Protein Intramembrane Processing by β and γ-Secretases
By onDegradation and recycling of plasma membrane proteins occur through the endolysosomal system. Endo-IP, captures early endosomes for proteomic analysis, revealing insights into protein trafficking and disease-related cargo like APP in Alzheimer's.
Unaltered T cell responses to common antigens in individuals with Parkinson’s disease
By onT cells have been shown to be overactive in individuals with PD. The authors tested a wide variety of commonly encountered immune targets on PD and non-PD control derived T cells and observed no differences between their immune responses.
Fecal metagenomic sequencing data for PD patients and controls from the BioCollective
By onFecal metagenomic sequencing data associated with Boktor et al. (2023). This dataset includes samples from the BioCollective cohort.
iPSC differentiation into Microglia
By onThis protocol describes iPSC differentiation into microglia.
Open science takes on Parkinson’s disease
By onThis article explains the Aligning Science Across Parkinson's (ASAP) initiative's commitment to open science by taking a deep look into how the initiative contributes to the open science movement.
Presynaptic autophagy is coupled to the synaptic vesicle cycle via ATG-9
By onAutophagy is a cellular degradation pathway essential for neuronal health and function. Autophagosome biogenesis occurs at synapses, is locally regulated, and increases in response to neuronal activity. The mechanisms that couple autophagosome biogenesis to synaptic activity remain unknown. In this study, we determine that trafficking of ATG-9, the only transmembrane protein in the core autophagy pathway, links the synaptic vesicle cycle with autophagy. ATG-9-positive vesicles in C. elegans are generated from the trans-Golgi network via AP-3-dependent budding and delivered to presynaptic sites. At presynaptic sites, ATG-9 undergoes exo-endocytosis in an activity-dependent manner. Mutations that disrupt endocytosis, including a lesion in synaptojanin 1 associated with Parkinson’s disease, result in abnormal ATG-9 accumulation at clathrin-rich synaptic foci and defects in activity-induced presynaptic autophagy. Our findings uncover regulated key steps of ATG-9 trafficking at presynaptic sites and provide evidence that ATG-9 exo-endocytosis couples autophagosome biogenesis at presynaptic sites with the activity-dependent synaptic vesicle cycle.
In Silico analysis links the NSL complex to Parkinson’s disease and the mitochondria – Protein-protein interaction data to functional enrichment analysis
By onA bioinformatics approach taken to investigate the interactome of the NSL complex, to unpick its relevance to Parkinson’s disease progression.
Fiber Photometry (Mouse)
By onThis protocol describes the procedure for fiber photometry in awake behaving mice. It includes details on the surgical implantation of fibers.
In situ architecture of the lipid transport protein VPS13C at ER–lysosome membrane contacts
By onLoss-of-function mutations in VPS13C are responsible for rare cases of familial early onset Parkinson’s disease. Using cryo-ET, the authors provide in-situ evidence for a bridge-model of VPS13 in lipid transport.
pCAG-OSF-ATG13 (2-197)-L151D
By onPlasmid for expression of human ATG13 HORMA L151D mutant in mammalian cells.
Measurement of GLP-1 release in cell supernatant from Hutu-80 enteroendocrine cells via ELISA
By onMeasurement of GLP-1 release in cell supernatant from Hutu-80 enteroendocrine cells via ELISA