Hippocampal Neuronal Culture
By onThis protocol describes the procedure for hippocampal neuronal cultures from new - born mouse pups.
Bile acids and neurological disease
By onThis review will focus on how bile acids are being used in clinical trials to treat neurological diseases due to their central involvement with the gut-liver-brain axis and their physiological and pathophysiological roles in both normal brain function and multiple neurological diseases. The synthesis of primary and secondary bile acids species and how the regulation of the bile acid pool may differ between the gut and brain is discussed. The expression of several bile acid receptors in brain and their currently known functions along with the tools available to manipulate them pharmacologically are examined, together with discussion of the interaction of bile acids with the gut microbiome and their lesser-known effects upon brain glucose and lipid metabolism. How dysregulation of the gut microbiome, aging and sex differences may lead to disruption of bile acid signalling and possible causal roles in a number of neurological disorders are also considered. Finally, we discuss how pharmacological treatments targeting bile acid receptors are currently being tested in an array of clinical trials for several different neurodegenerative diseases
Mass spectrometry proteomic data supporting the PPM1H/Rab8A crosslinking study described in doi: 10.15252/embr.202152675
By onRaw data associated with DOI: 10.15252/embr.202152675
LC3-lipidation-assay
By onProtocol for an in vitro LC3 lipidation assay using purified proteins and synthetic liposomes.
pCAG-OSF-ATG13 (2-197)-L151D
By onPlasmid for expression of human ATG13 HORMA L151D mutant in mammalian cells.
Measurement of GLP-1 release in cell supernatant from Hutu-80 enteroendocrine cells via ELISA
By onMeasurement of GLP-1 release in cell supernatant from Hutu-80 enteroendocrine cells via ELISA
Structural basis for the specificity of PPM1H phosphatase for Rab GTPases
By onRaw data associated with DOI: 10.15252/embr.202152675
pU6-pegRNA-LRRK2-G2019S-3a
By onIt can be used to introduce LRRK2-G2019S mutation using prime editing.
Sample Collection and Processing for RNA Analysis
By onThis protocol delineates the steps taken to collect frozen postmortem human brain samples and process them for RNA extraction and analysis.
Single-molecule Immunofluorescence Tissue Staining Protocol for Oligomer Imaging V.3
By onThis protocol details background fluorescence quenching and immunofluorescence staining of human brain tissue for oligomer imaging.
Protocols for “Characterizing the diversity of enteric neurons using Dopamine Transporter (DAT)-Cre reporter mice”.
By onThis is a collection of protocols associated to the manuscript "Characterizing the diversity of enteric neurons using Dopamine Transporter (DAT)-Cre reporter mice".
Creating pooled CRISPR-Cas9 knock-outs in NIH-3T3 cells
By onTo validate a genome wide CRISPR screen, the authors select the top hits and create lentiviruses to validate the hits.
Western blot – alpha-synuclein
By onThis protocol describes how to detect alpha-synuclein in protein derived from STC-1 cells by western blot using DAB/peroxidase or ECL to visualize the bands.
