Optogenetic Manipulation (Mouse)
By onThis protocol describes the steps for in vivo ontogenetic manipulation in mice, including assembly of fiber-ferrules, surgical implantation of fibers, and testing procedure.
cDNA clone for bacterial expression of human PPM1H H153D
By onPlasmid for bacterial expression of human PPM1H (H153D); contains TEV protease site for removal of 6His tag.
Brain processing, slicing and immunohistochemistry protocol
By onThis protocol is a step by step procedure from collecting brain samples to immunohistochemical staining and mounting brain slices.
Transcriptional analysis of peripheral memory T cells reveals Parkinson’s disease-specific gene signatures–Cell Surface FCS
By onFCS files corresponding to the cell surface experiment described in "Transcriptional analysis of peripheral memory T cells reveals Parkinson’s disease-specific gene signatures"
CRISPR tagging of the EEA1 gene in H9 ES cells for Endo-IP
By onProtocol for tagging EEA1 gene in human H9 ES cells with a 3X flag using CAS9 and oligonucleotide template is described.
The AAA+ chaperone VCP disaggregates Tau fibrils and generates aggregate seeds in a cellular system
By onProtein aggregates, like tau, are associated with neurodegenerative diseases. The authors show that the AAA+ chaperone, VCP, is recruited to ubiquitylated Tau, resulting in disaggregation.
The SATB1-MIR22-GBA axis mediates glucocerebroside accumulation inducing a cellular senescence-like phenotype in dopaminergic neurons
By onStudy shows SATB1, MIR22HG, and GBA genes form a pathway in Parkinson's Disease. Dysregulation leads to GluCer accumulation, causing cellular senescence in dopaminergic neurons, potentially explaining neuroinflammation seen in PD and aging.
Pole Test to assess motor coordination in parkinsonian mice
By onThe pole test evaluates the motor coordination ability of a mouse by having them grasp a pole and then descend to its home cage.
Mouse Ovariectomy
By onThis protocol describes the steps to perform an ovariectomy in a mouse. This procedure is used to induce menopause.
Motor learning selectively strengthens cortical and striatal synapses of motor engram neurons
By onIn this study, Hwang, Roth, et al. find that motor learning recruits a population of engram neurons in the motor cortex that are reactivated during task performance. Motor learning leads to selective remodeling of dendritic spines and strengthening of outputs to the striatum of M1 engram neurons.
Border-associated macrophages mediate the neuroinflammatory response in an alpha-synuclein model of Parkinson disease
By onBorder-associated macrophages (BAMs) are crucial in Parkinson's disease pathogenesis by initiating neuroinflammation, highlighting a potential target for therapeutic intervention.
Mouse reaching task
By onThe repository includes materials for building and analyzing data from a mouse-reaching task: a bill of materials, 3D models, schematics, an Arduino controller, video recording scripts, and data analysis scripts using DLC.
iPS Cell line: CRICKi012-A (iFCI017), c.G152A mutation in Exon 3 of SNCA
By onMutations in SNCA gene cause rare Parkinson’s disease. iPSC lines from individuals with SNCA G51D mutation were generated successfully, showing normal characteristics. These iPSC lines can aid in studying synucleinopathies for potential therapies.
CD3 Cell Density in Substantia Nigra and Cerebral Peduncle Image Analysis
By onThis protocol describes how to measure CD3 density in the substantia nigra and cerebral peduncle using haematoxylin and DAB-stained brain sections.
Yeast cells live fluorescence imaging
By onProtocol for sample preparation and live fluorescence imaging of yeast cells.
Preparing samples for NGS
By onThis protocol describes a standard procedure used to prepare PCR samples for Next Generation Sequencing (NGS).
Unaltered T cell responses to common antigens in individuals with Parkinson’s disease
By onT cells have been shown to be overactive in individuals with PD. The authors tested a wide variety of commonly encountered immune targets on PD and non-PD control derived T cells and observed no differences between their immune responses.
Mitochondrial Antigen Presentation in RAW macrophages
By onThis protocol details methods for 3-day Mitochondrial Antigen Presentation Assay in a murine macrophage cell line (RAW) that expresses a glycoprotein B (gB) of herpes simplex virus 1 (HSV1) targeted to the mitochondrial matrix (mito-gB). A gB-specific CD8+ T cell hybridoma recognizing the gB498–505peptide loaded on MHC class I molecules is also used to monitor antigen presentation through a beta-galactosidase assay kit.
