Striatal Cannula Window Implantation
By onThis protocol describes how to implant a cannula window over the dorsolateral striatum in mice to enable two-photon imaging of dopamine axons in the dorsal striatum.
Workflow for proteomic analysis of purified lysosomes in cells lacking GRN V.3
By onAssociated with the following preprint (published September 30th 2021): Progranulin deficiency results in reduced bis(monoacylglycero)phosphate (BMP) levels and gangliosidosis Sebastian Boland, Sharan Swarup, Yohannes A. Ambaw, Ruth C. Richards, Alexander W. Fischer, Shubham Singh, Geetika Aggarwal, Salvatore Spina, Alissa L. Nana, Lea T. Grinberg, William W. Seeley, Michal A. Surma, Christian Klose, Joao A. Paulo, Andrew D. Nguyen, J. Wade Harper, Tobias C. Walther, Robert V. Farese Jr. bioRxiv 2021.09.30.461806; doi: https://doi.org/10.1101/2021.09.30.461806
Tet-off TauRD (HEK293T)
By onAssociated with the following preprint: Saha et al., 2022 (published on biorxiv on Feb 19th, 2022) https://www.biorxiv.org/content/10.1101/2022.02.18.481043v1.full
Expression and purification protocol of GST-TAX1BP1
By onThis protocol details the expression and purification protocol of GST-TAX1BP1.
pCAG- WIPI2dR108E- cs-TEV -STREP
By onPlasmid for mammalian expression of human WIPI2d R108E with C-terminal Strep.
pAC150-Keima-REEP5
By onSummary: Piggybac vector used to express Keima-REEP5 autophagy flux reporter, a tool to monitor autophagy flux in cells by measuring pH changes in autophagosomes and lysosomes.
Acute striatal or midbrain fiber photometry in head-fixed mice
By onWhile this protocol focuses recordings from striatum with GCaMP, it can be easily modified to record from other brain regions and with other fluorescent reporters.
Standard Operating Procedure: Mouse transcardiac perfusion protocol
By onMouse transcardiac perfusion protocol and storage.
Automated Immunohistochemistry Staining
By onThe protocols describe the steps for automated immunohistochemistry staining using the Ventana Discovery® XT Immunostainer.
iPS Cell line CRICKi011-A (iFCI016), c.G152A mutation in Exon 3 of SNCA
By onMutations in the SNCA gene cause rare autosomal dominant Parkinson’s disease. iPSC lines were created from individuals with SNCA G51D mutations, showing potential for disease modeling and therapy development for synucleinopathies.
In vitro kinase assay: phosphorylation of PI3Kc1 by TBK1and ULK1
By onProtocol describing in vitro kinase assay, in which PI3Kc is tested for phosphorylation by TBK1 and ULK1 complex either by western blotting (S29 on ATG14L subunit) or mass spectrometry analysis.
H9 ES AAVS1-NGN2 FAM134A-/-; PiggyBac-Keima-RAMP4
By onES cells were modified to create iNeurons lacking FAM134A and expressing Keima-RAMP4 ER-phagy flux reporter. CRISPR/Cas9 was used to introduce NEUROG2 construct in AAVS1 locus. Transfected with Keima-RAMP4 vector.
Temporal landscape of mitochondrial proteostasis governed by the UPRmt
By onThe authors developed a functional proteomics framework, termed MitoPQ (Mitochondrial Proteostasis Quantification), to dissect the UPRmts role in maintaining proteostasis during stress.
Transcriptional analysis of peripheral memory T cells reveals Parkinson’s disease-specific gene signatures–Gene Validation FCS
By onFCS files corresponding to the gene validation experiment described in "Transcriptional analysis of peripheral memory T cells reveals Parkinson’s disease-specific gene signatures"
Preparation of mouse embryonic fibroblast (MEF) feeder plates for hPSC cultures
By onThis protocol describes the preparation of mouse embryonic fibroblasts (MEFs) as feeder cells for human pluripotent stem cell (hPSC) culture.
Immunofluorescence staining for postmortem mouse brain tissue
By onThis is a basic protocol for staining mouse brain tissues using immunofluorescence and immunohistochemistry techniques.
Optogenetic Manipulation (Mouse)
By onThis protocol describes the steps for in vivo ontogenetic manipulation in mice, including assembly of fiber-ferrules, surgical implantation of fibers, and testing procedure.
