Structural conversion of α-synuclein at the mitochondria induces neuronal toxicity
By onOur study highlights intracellular conformational changes as a mechanism of de novo α-Syn oligomerization at mitochondrial membranes and subsequent neuronal toxicity, also observed in iPSC-derived neurons with A53T mutations from patients with PD.
Protocol for Image processing and analysis of VPS13D recruitment to mitochondria
By onThis protocol details the image processing and analysis of VPS13D recruitment to mitochondria as it was performed in https://doi.org/10.1083/jcb.202010004. The first part details the analysis of acute optogenetic recruitment, and the second part of basal recruitment under different conditions.
Single-cell transcriptomic and proteomic analysis of Parkinson’s disease Brains
By onThis article established a single-nucleus transcriptomic profile of the prefrontal cortex from postmortem human brains of six patients with PD and six age-matched controls.
HeLa culture, transfection, and labeling of Halo-fusion proteins
By onProtocol for HeLa culture, transfection, and labelling of Halo-fusion proteins.
Surgical procedures for simultaneous optogenetic manipulation in the SNc and acute fiber photometry in the dorsal striatum
By onThis protocol describes the three surgical procedures required for simultaneous optogenetic stimulation in the Substantia Nigra pars compacta (SNc) and acute fiber photometry in the dorsal striatum.
Midbrain-like Organoids generation from hiPSCs
By onProtocol for generating midbrain organoids from human iPSCs.
pCAG-OSF-ATG13 (2-197)-R153D
By onPlasmid: Expresses human ATG13 HORMA R153D mutant in mammalian cells.
Diabetes and neuroaxonal damage in Parkinson’s disease
By onOur findings affirm Uyar et al's report of an association between PD-DM and more severe neuroaxonal damage. T2DM and PD share several pathological processes, in par mediated by hyperglycemia and possibly diabetic neuropathy.
Essential surgeries for the electrophysiological recording from a behaving non-human primate brain 2 (Craniotomy)
By onProtocol for performing a craniotomy in a recording chamber on a cranial implant in nonhuman primates.
Isolation of NeuN+ cells from brain tissue (for CUT and RUN)
By onThis protocol describes the steps to isolate NeuN+ cells from brain tissue in preparation for CUT and RUN.
Synaptic Location Is a Determinant of the Detrimental Effects of α-Synuclein Pathology to Glutamatergic Transmission in the Basolateral Amygdala
By onStudy explores α-synuclein's impact on amygdala in Parkinson's. Aggregation affects cortico-BLA transmission, linked to psychiatric deficits in PD.
Passaging of feeder-free hPSCs
By onThis protocol describes the process of passaging human pluripotent stem cells (hPSCs) for feeder-free culturing of hPSCs using Accutase or ReLeSF.
fMRI tasks to explore emotion-motor interactions
By onCode for fMRI tasks designed to explore emotion-motor interactions—Active Escape and Approach-Avoidance tasks in the Socal Kinesia and Incentivization for Parkinson's Disease (SKIP) dataset.
Assay for Dual Rab GTPase binding to the LRRK2 Armadillo Domain
By onThe LRRK2 Armadillo domain contains multiple Rab GTPase binding sites. This assay shows that the sites can be occupied simultaneously.
CURTAIN—A unique web-based tool for exploration and sharing of MS-based proteomics data
By onLinks can also be reported in publications allowing readers to further survey the reported data. The authors discuss benefits for the research community of publishing proteomic data containing a shareable web-link.
