The SATB1-MIR22-GBA axis mediates glucocerebroside accumulation inducing a cellular senescence-like phenotype in dopaminergic neurons
By onStudy shows SATB1, MIR22HG, and GBA genes form a pathway in Parkinson's Disease. Dysregulation leads to GluCer accumulation, causing cellular senescence in dopaminergic neurons, potentially explaining neuroinflammation seen in PD and aging.
Pole Test to assess motor coordination in parkinsonian mice
By onThe pole test evaluates the motor coordination ability of a mouse by having them grasp a pole and then descend to its home cage.
HyDrop Bead Generation & PCR Barcoding v1.0
By onProtocol for producing dissolvable barcoded hydrogel beads used in HyDrop experiments.
The AAA+ chaperone VCP disaggregates Tau fibrils and generates aggregate seeds in a cellular system
By onProtein aggregates, like tau, are associated with neurodegenerative diseases. The authors show that the AAA+ chaperone, VCP, is recruited to ubiquitylated Tau, resulting in disaggregation.
Preparing samples for NGS
By onThis protocol describes a standard procedure used to prepare PCR samples for Next Generation Sequencing (NGS).
CD3 Cell Density in Substantia Nigra and Cerebral Peduncle Image Analysis
By onThis protocol describes how to measure CD3 density in the substantia nigra and cerebral peduncle using haematoxylin and DAB-stained brain sections.
Yeast cells live fluorescence imaging
By onProtocol for sample preparation and live fluorescence imaging of yeast cells.
shoebridges/asap_phosphosites: Preview Release
By onSource data associate with publication "Unconventional Initiation of PINK1/Parkin Mitophagy by Optineurin"
Glucocerebrosidase 1 and leucine-rich repeat kinase 2 in Parkinson disease and interplay between the two genes
By onReview: This review focuses on the endolysosomal pathway roles of GBA and LRRK2, highlighting the role and activity of Rab GTPases as LRRK2 substrates.
Mitochondrial Antigen Presentation in RAW macrophages
By onThis protocol details methods for 3-day Mitochondrial Antigen Presentation Assay in a murine macrophage cell line (RAW) that expresses a glycoprotein B (gB) of herpes simplex virus 1 (HSV1) targeted to the mitochondrial matrix (mito-gB). A gB-specific CD8+ T cell hybridoma recognizing the gB498–505peptide loaded on MHC class I molecules is also used to monitor antigen presentation through a beta-galactosidase assay kit.
Early Endosome Capture Proteomics and its Application to Amyloid Precursor Protein Intramembrane Processing by β and γ-Secretases
By onDegradation and recycling of plasma membrane proteins occur through the endolysosomal system. Endo-IP, captures early endosomes for proteomic analysis, revealing insights into protein trafficking and disease-related cargo like APP in Alzheimer's.
Unaltered T cell responses to common antigens in individuals with Parkinson’s disease
By onT cells have been shown to be overactive in individuals with PD. The authors tested a wide variety of commonly encountered immune targets on PD and non-PD control derived T cells and observed no differences between their immune responses.
Fecal metagenomic sequencing data for PD patients and controls from the BioCollective
By onFecal metagenomic sequencing data associated with Boktor et al. (2023). This dataset includes samples from the BioCollective cohort.
iPSC differentiation into Microglia
By onThis protocol describes iPSC differentiation into microglia.
