Stereotaxic injection of viral vectors
By Julia Julia Leonard onStereotaxic injection of viral vectors.
Protocol for ex vivo patch clamping
By Julia Julia Leonard onProtocol for ex vivo patch clamping Protocol for ex vivo patch clamping.
Brain processing, slicing, and immunohistochemistry protocol
By Julia Julia Leonard onThis protocol is a step by step procedure from collecting brain samples to immunohistochemical staining and mounting brain slices.
Handling and behavioral training protocol for in vivo electrophysiological and optogenetic manipulation of basal ganglia neurons in awake head-fixed mice
By Julia Julia Leonard onThis protocol details the habituation of animals and behavioral training of animals for in vivo electrophysiology or optogenetic experiments.
Confocal imaging on Nikon AXR confocal microscope
By Julia Julia Leonard onThis protocol provides a step by step protocol to acquire images on Nikon AXR confocal set-up.
Immunofluorescence staining
By Julia Julia Leonard onThe protocol describes immunofluorescence staining on brain sections.
Automated immunohistochemistry staining
By Julia Julia Leonard onThe protocols describe the steps for automated immunohistochemistry staining using the Ventana Discovery® XT Immunostainer.
Luminescent conjugated oligothiophenes (LCO) staining
By Julia Julia Leonard onThe protocol describes Luminescent Conjugated Oligothiophenes staining on brain sections.
Protocol Collection: Combinatorial selective ER-phagy remodels the ER during neurogenesis
By Julia Julia Leonard onThis molecular inventory of ER proteome remodeling and versatile genetic toolkit provides a quantitative framework for understanding contributions of individual ER-phagy receptors for reshaping ER during cell state transitions.
Lentivirus preparation for neuronal transdifferentiation
By Julia Julia Leonard onThis is a protocol for the preparation of Lentiviruses.
Human primary fibroblast culture
By Julia Julia Leonard onThis is a protocol for human primary fibroblast culture.
Neuronal transdifferentiation from human primary adult fibroblasts
By Julia Julia Leonard onThis is a protocol for the direct conversion of human primary fibroblasts into neurons using a combination of transcription factor- and small molecule-based approach. The majority of converted neurons showing characteristics of cortical glutamatergic neurons.
Cytokine profiling analysis on conditioned medium of human neurons using Luminex multiplex assay
By Julia Julia Leonard onThis protocol is used to identify secreted inflammatory factors by cytokine profiling of the conditioned medium from human transdifferentiated neurons of healthy donors and AD patients.
Immunohistochemistry on free-floating and paraffin-embedded tissue sections
By Julia Julia Leonard onThis protocol is used for free-floating frozen (30-50 microns) and paraffin-embedded (10 microns) tissue sections.
Proteome of R1441C and G2019S LRRK2 knock in mice after striatal motor learning task
By Julia Julia Leonard onTo identify the signaling pathways that underlie the motor learning deficits, specifically in the RC mice, the authors performed six-plex tandem mass tag (TMT) quantitative mass spectrometry (MS) to compare the protein expression of either RC or GS and WT mice following the five days of the rotarod test.
Tabular data of fast-scanning voltammetry and physiology experiments in LRRK2 KI mice
By Julia Julia Leonard onThe tabular data of fast-scanning cyclic voltammetry and physiology experiments from manuscript: "R1441C and G2019S LRRK2 knockin mice have distinct striatal molecular, physiological, and behavioral alterations." by Xenias et al.
Tabular data of striatal motor learning experiments in LRRK2 KI mice
By Julia Julia Leonard onThe tabular data of the behavioral experiments described in the manuscript titled, "R1441C and G2019S LRRK2 knockin mice have distinct striatal molecular, physiological, and behavioral alterations."
Fast scan cyclic voltammetry
By Julia Julia Leonard onThis protocol describes the ex vivo fast-scan cyclic voltammetry (FSCV) technique for detecting dopamine in the dorsal striatum in mice.
Behavioral tests in rodents coupled with dopamine signaling manipulations
By Julia Julia Leonard onThese two protocols describe behavioral tests associated with Parkinson’s disease phenotypes in mice. The striatal motor learning protocol (rotarod test) is a two-phase paradigm assessing striatal motor learning under reversible dopamine antagonism.
