Intramuscular PFF injection
By Emma Sherrell onThis protocol details the intramuscular PFF injection procedure. This protocol details the intramuscular PFF injection procedure.
Synthesis of 1-(3-Chlorophenethyl)-3-cyclopentylpyrimidine-2,4,6-(1H,3H,5H)-trione
By Julia Julia Leonard on1-(3-chlorophenethyl)-3-cyclopentylpyrimidine-2,4,6-(1H,3H,5H)-trione (8) is a potent and highly selective Ca(V)1.3 L-type calcium channel antagonist. This protocol is an adaption of Kang et al., 2012's method of the synthesis of this chemical.
Open field
By Emma Sherrell onThis protocol details about Open Field procedure. This protocol details about Open Field procedure.
A fluid-walled microfluidic platform for human neuron microcircuits and directed axotomy
By Julia Julia Leonard onPublished: Cortical axons in conduits are severed by a media jet; then, brain-derived neurotrophic factor and striatal neurons in distal chambers promote axon regeneration. As additional conduits and chambers are easily added, this opens up the possibility of mimicking complex neuronal networks and screening drugs for their effects on connectivity. View original preprint.
AAV injection in the nodose ganglia of mouse
By Emma Sherrell onThe study of these ganglia is challenging in small animals due to size and location. Hence, in this protocol, the team describes a practical surgical approach to the vagal trunk, and the JNG complex in mice.
Automatic flow in fluid-walled dumbbells driven by Laplace pressure
By Julia Julia Leonard onThis protocol describes experiments performed to quantify pressure and volume variations inside fluid-walled dumbbells when a pressure difference is generated between the two chambers. Difference in Laplace pressure automatically flows medium from the high-pressure chamber to the low pressure one. Flow stops when pressures are equilibrated.
Immunostaining of corticostriatal culture on fluid-walled dumbbells
By Julia Julia Leonard onThis protocol describes the immunocytochemistry of the iPSC-derived corticostriatal culture on fluid-walled dumbbells.
Fura-2 imaging of ionomycin response, with and without R568; a CaSR positive modulator
By Julia Julia Leonard onFura-2 imaging protocol to accompany Kilfeather, Khoo et al., 2023: Single cell spatial transcriptomic and translatomic profiling of dopaminergic neurons in health, aging, and disease.
Generation of stable cell lines using retroviral system
By Emma Sherrell onThis protocol details generation of stable cell lines using retroviral system.
Immunocytochemistry for CASR in iPSc-derived dopaminergic neurons
By Julia Julia Leonard onCASR immunocytochemistry protocol to accompany Kilfeather, Khoo et al., 2023: Single cell spatial transcriptomic and translatomic profiling of dopaminergic neurons in health, aging, and disease.
Assessing autophagy using the HaloTag-LC3B cleavage assay
By Emma Sherrell onAssay to detect HaloTag-LC3B during starvation autophagy based on https://doi.org/10.7554/eLife.78923.
DAT-TRAP Protocol
By Julia Julia Leonard onThis protocol describes the capture of eGFP-L10a-tagged ribosomes and mRNA from DAT-expressing cells in mouse ventral midbrain.
Fast-scan cyclic voltammetry to assess dopamine release in ex vivo mouse brain slices
By Julia Julia Leonard onThis protocol is to assess whether a drug changes the dopamine concentration released following a single pulse (1p) electrical stimulation.
Three-step docking by WIPI2, ATG16L1 and ATG3 delivers LC3 to the phagophore: Molecular dynamics simulation dat
By Emma Sherrell onAtomistic molecular dynamics simulation data set accompanying manuscript "Three-step docking by WIPI2, ATG16L1 and ATG3 delivers LC3 to the phagophore."
Peripheral MC1R activation modulates immune responses and is neuroprotective in a mouse model of Parkinson’s disease
By Julia Julia Leonard onPreprint: The present study investigates the impact of NDP-MSH, a synthetic melanocortin receptor (MCR) agonist that does not cross BBB, on the immune system and the nigrostriatal dopaminergic system in mouse model of PD.
Raw band intensity values for ATG3 WT or Mutants in vitro LC3 lipidation
By Emma Sherrell onRaw band intensity values used for the quantification of in vitro LC3 lipidation results (Fig4C) in Three-step docking by WIPI2, ATG16L1 and ATG3 delivers LC3 to the phagophore.
Raw band intensity values for Halo-LC3B
By Emma Sherrell onRaw band intensity values used for the quantification of percentage autophagy levels.
Whole mount dissection and staining of enteric nervous system
By Julia Julia Leonard onThis protocol details whole mount dissection and staining of the enteric nervous system.
Protocol for in vivo electrophysiological and optogenetic manipulation of basal ganglia neurons in awake head-fixed mice
By Julia Julia Leonard onStep by step procedure for in vivo electrophysiological and optogenetic manipulation of basal ganglia neurons in awake head-fixed mice.
Headplate surgery protocol for in vivo electrophysiological and optogenetic manipulation of basal ganglia neurons in awake head-fixed mice
By Julia Julia Leonard onStep by step protocol for headplate surgery for in vivo electrophysiological and optogenetic manipulation of basal ganglia neurons in awake mice.
