Standard operating procedure (SOP) for combined ICA and systemic administration of MPTP: The overlesioned (Bilateral Asymmetric) non-human primate model
By Emma Sherrell onThis SOP describes the procedures for the combined internal carotid artery (ICA) and systemic (intravenous or intramuscular) administration of MPTP in non-human primates. This model produces an overlesioned or bilateral asymmetric model, induced by unilateral ICA plus systemic administration. This protocol has been adapted from Bankiewicz et al. (1999).
Electrophysiological recording from a chronic chamber-implanted non-human primate
By Emma Sherrell onThis protocol describes the preparation and insertion of extracellular recording electrodes into intracranial targets of a non-human primate implanted with a chronic recording chamber. A piercing guide cannula is used to penetrate the dura and to guide the electrode. Practices related to targeting, electrode cleaning, and electrode movement and settling relative to brain tissue are discussed.
Essential surgeries for the electrophysiological recording from a behaving non-human primate brain 2 (Craniotomy)
By Emma Sherrell onOutlined are the steps to complete a craniotomy in an existing recording chamber placed on a cranial implant. This procedure can be done concurrently with the implant surgery (not recommended), or can take place upon recovery from the implant surgery.
Essential surgeries for the electrophysiological recording from a behaving non-human primate brain 1 (Head fixation post and recording chamber implantation)
By Emma Sherrell onThe performing surgical procedures under strictly sterile conditions is the key to the successful progress of the project.
Maintenance of the recording chamber for non-human primate (Chamber cleaning and dura scraping)
By Emma Sherrell onOnce a craniotomy has been done in the chamber, the inside of the chamber must be cleaned regularly (2-3 times per week) to prevent infection. This protocol explains two procedures to maintain the recording chamber in a healthy condition:(1) chamber cleaning and (2) dura scraping.
Evaluation of a novel method for spectral analysis of neurophysiology data
By Emma Sherrell onThis code implements a novel method for correcting a distortion effect that is present in the power spectra of neuronal spike trains. This method is compared to an alternative, inter-spike interval shuffling procedure that has been conventionally employed for this purpose in the past.
Synchronous measurements of extracellular action potentials and neurochemical activity with carbon fiber electrodes in nonhuman primates
By Emma Sherrell onPublished: Synchronous measures of spike and dopamine signals displayed contrasting relations to the behavioral task parameters, as taken from the small set of representative data, suggesting a complex relationship between these two modes of neural signaling. View original preprint.
Neuropathological assessment of the olfactory bulb and tract in individuals with COVID-19
By Emma Sherrell onPublished: The team concluded from their autopsy series that after a fatal course of COVID-19, microscopic changes, when present, in the rostral, intracranial portion of the olfactory circuitry generally reflected neurodegenerative processes seen elsewhere in the brain. In general, inflammation correlated best with the degree of Alzheimer’s-linked tauopathy and declined with progression of age in COVID19+ patients. View original preprint.
Persistent hyposmia as surrogate for α-Synuclein-Linked brain pathology
By Emma Sherrell onPreprint: The team identified significant age- and posit that simple-to-administer, quantitative smell tests could serve as inexpensive screening tools in future population studies for the identification of α-synuclein-related brain disorders, including Parkinson's during its premotor phase.
Response to: “Is Gauchian genotyping of GBA1 variants reliable?”
By Emma Sherrell onPreprint: To understand the cause of these discrepancies, the team reviewed their data, and concluded that they are misinterpreting Gauchian results in 8 of the 11 discrepant samples, and incorrectly using Gauchian to analyze low-coverage 1kGP samples.
Population fraction of Parkinson’s disease attributable to preventable risk factors
By Emma Sherrell onThe complete R-based Posit project reproduces the analyses and output in the article, "Population fraction of Parkinson's disease attributable to preventable risk factors."
Metagenomics of Parkinson’s disease implicates the gut microbiome in multiple disease mechanisms
By Emma Sherrell onThe code provided here is the same "Supplementary Code" that is provided in the supplement of the manuscript. Individual level raw shotgun sequences and metadata are available on NCBI Sequence Read Archive (SRA) under BioProject ID PRJNA834801.
Characterizing dysbiosis of the Parkinson’s disease gut microbiome using shotgun metagenomics
By Emma Sherrell onTo characterize the dysbiosis at species, gene family, and metabolic pathway resolution, the authors enrolled 490 PD and 234 age and geographically matched neurologically healthy individuals, collected stool and metadata, and conducted deep shotgun metagenomic sequencing of fecal DNA. Sequences and metadata are provided here.
Microbial amyloids in neurodegenerative amyloid diseases
By Emma Sherrell onPublished: Inhibiting microbial amyloids or their interactions with the host, may therefore represent a tangible target to limit various amyloid pathologies.
Anionic nanoplastic contaminants promote Parkinson’s disease–associated α-synuclein aggregation
By Emma Sherrell onThese results highlight a potential link for further exploration between nanoplastic pollution and α-synuclein aggregation associated with Parkinson’s disease and related dementias.
A leaky gut dysregulates gene networks in the brain associated with immune activation, oxidative stress, and myelination in a mouse model of colitis
By Emma Sherrell onPreprint: The team provides a comprehensive evaluation of multiple systems in a prevalent experimental model of intestinal permeability, which will inform future studies using this model and others, assist in the identification of druggable targets in the gut-brain axis, and contribute to our understanding of the concomitance of intestinal and neuropsychiatric dysfunction.
The R1441C-LRRK2 mutation induces myeloid immune cell exhaustion in an age- and sex-dependent manner
By Emma Sherrell onPreprint: LRRK2 has become an appealing target in PD, but findings suggest that more research is required to understand the cell-type-specific consequences and optimal timing of LRRK2-targeting therapeutics.
Calcium influx into astrocytes plays a pivotal role in inflammation-driven behaviors
By Emma Sherrell onGraphical abstract.
CryoEM structures of amplified alpha-synuclein fibril class A type I with extended core from DLB case I
By Emma Sherrell onClassification: PROTEIN FIBRIL Organism(s): Homo sapiens Expression System: Escherichia coli BL21(DE3) Mutation(s): No
CryoEM structure of amplified alpha-synuclein fibril class A type I with compact core from DLB case III
By Emma Sherrell onClassification: PROTEIN FIBRIL Organism(s): Homo sapiens Expression System: Escherichia coli 'BL21-Gold(DE3)pLysS AG Mutation(s): No
