Orchestration of selective autophagy by cargo receptors
By onThe authors review recent insights into the mechanisms of action of cargo receptors in selective autophagy by focusing on the roles of sequestosome-like cargo receptors in the degradation of misfolded, ubiquitinated proteins and damaged mitochondria.
Gene editing of YIPF4, CALCOCO1, and ATG7 in HeLa cells
By onThe text outlines the process of generating YIPF4, FIP200, and ATG7 knockout cell lines in HeLa cells through CRISPR-Cas9 technology.
Impact of 100 LRRK2 variants linked to Parkinson’s Disease on kinase activity and microtubule binding
By onThis study systematically investigates 100 LRRK2 variants linked to PD, and provides rationale for variant carrier inclusion/exclusion in ongoing and future LRRK2 inhibitor clinical trials.
Microscopy-based evaluation of Parkin translocation and mitophagy in FBXO7-/- cell lines
By onAssociated with preprint:
Genome-wide Analysis of Motor Progression in Parkinson Disease
By onThe genetic basis of Parkinson disease (PD) motor progression is largely unknown. Previous studies of the genetics of PD progression have included small cohorts and shown a limited overlap with genetic PD risk factors from case-control studies. Here, we have studied genomic variation associated with PD motor severity and early-stage progression in large longitudinal cohorts to help to define the biology of PD progression and potential new drug targets.
Loss of tau expression attenuates neurodegeneration associated with α-synucleinopathy
By onThe authors examine the role of tau in the onset and progression of αS pathology using a transgenic mouse model of α-synucleinopathy lacking mouse tau expression.
pU6-pegRNA-HEK3-CTTins-px330-scaffold
By onPlasmid: This resource can be used to introduce HEK3 CTT insertion using prime editing. It serves as a good positive control for prime editing.
Structure of human ULK1 complex core (2:2:2 stoichiometry) of the ATG13(450-517) mutant
By onStructure of human ULK1 complex core (2:2:2 stoichiometry) of the ATG13(450-517) mutant
Transferrin uptake assay to measure Clathrin-mediated endocytosis in HIPCS derived neurons
By onThis protocol is used to measure Transferrin uptake as a way to investigate Clathrin-mediated endocytosis in HIPCS derived neurons, or other cell types.
Code for making forest plots for top GWAS loci
By onThis repository contains the script used to create forest plots for top loci .
Duplicating 96-well plate-cultured hPSCs clones
By onThis protocol describes a standard procedure for duplicating 96-well plate-cultured human pluripotent stem cells (hPSCs).
Primary data associated with doi: 10.1101/2022.04.25.489459 (A Feed-forward Pathway Drives LRRK2 kinase Membrane Recruitment and Apparent Activation)
By onRaw immunoblotting data; tabular data for quantifications; immunofluorescence images.
Immunostaining of iPSC-derived neurons
By onThe authors fix, permeabilize, and stain human iPSC-derived neurons for the purpose of observing and quantifying somal and axonal abundance of proteins of interest.
In Vivo Carbon Fiber Electrode Thread (CFET) Implantation and Testing Procedures
By onProcedures for implanting and testing CFET devices in rats are described.
Microscopy-based mitochondrial morphology measurements in iNeurons
By onAssociated with preprint: https://www.biorxiv.org/content/10.1101/2022.11.02.514817v1
pAC150-Keima-RAMP4
By onThe text describes the use of a PiggyBac vector to express the Keima-RAMP4 autophagy flux reporter, a tool for studying autophagy processes in cells.
